GCC2 As a New Early Diagnostic Biomarker for Non-Small Cell Lung Cancer

Overview

Journal Cancers (Basel)

Publisher MDPI

Specialty Oncology

Date 2021 Nov 13

PMID 34771645

Citations 9

Authors

Hyesun Jeong

Byeong Hyeon Choi

Jina Park

Jik-Han Jung

Hyunku Shin

Ka-Won Kang

Yu Hua Quan

Jewon Yu

Ji-Ho Park

Yong Park

Yeonho Choi

Hyun Koo Kim

SungHoi Hong

Affiliations

Soon will be listed here.

Abstract

No specific markers have been identified to detect non-small cell lung cancer (NSCLC) cell-derived exosomes circulating in the blood. Here, we report a new biomarker that distinguishes between cancer and non-cancer cell-derived exosomes. Exosomes isolated from patient plasmas at various pathological stages of NSCLC, NSCLC cell lines, and human pulmonary alveolar epithelial cells isolated using size exclusion chromatography were characterized. The GRIP and coiled-coil domain-containing 2 (GCC2) protein, involved in endosome-to-Golgi transport, was identified by proteomics analysis of NSCLC cell line-derived exosomes. GCC2 protein levels in the exosomes derived from early-stage NSCLC patients were higher than those from healthy controls. Receiver operating characteristic curve analysis revealed the diagnostic sensitivity and specificity of exosomal GCC2 to be 90% and 75%, respectively. A high area under the curve, 0.844, confirmed that GCC2 levels could effectively distinguish between the exosomes. These results demonstrate GCC2 as a promising early diagnostic biomarker for NSCLC.

Citing Articles

Molecular Biology of Cancer-Interplay of Malignant Cells with Emerging Therapies.

Boussios S, Sheriff M, Ovsepian S Int J Mol Sci. 2024; 25(23).

PMID: 39684799 PMC: 11642048. DOI: 10.3390/ijms252313090.

GCC2 promotes non-small cell lung cancer progression by maintaining Golgi apparatus integrity and stimulating EGFR signaling pathways.

Kim M, Jeong H, Choi B, Park J, Shin G, Jung J Sci Rep. 2024; 14(1):28926.

PMID: 39572606 PMC: 11582359. DOI: 10.1038/s41598-024-75316-1.

Extracellular vesicles in non-small cell lung cancer stemness and clinical applications.

Pandya P, Al-Qasrawi D, Klinge S, Justilien V Front Immunol. 2024; 15:1369356.

PMID: 38765006 PMC: 11099288. DOI: 10.3389/fimmu.2024.1369356.

Potential Avenues for Exosomal Isolation and Detection Methods to Enhance Small-Cell Lung Cancer Analysis.

Afridi W, Strachan S, Kasetsirikul S, Singh Pannu A, Soda N, Gough D ACS Meas Sci Au. 2023; 3(3):143-161.

PMID: 37360040 PMC: 10288614. DOI: 10.1021/acsmeasuresciau.2c00068.

Research progress of extracellular vesicles as biomarkers in immunotherapy for non-small cell lung cancer.

Ge Y, Ye T, Fu S, Jiang X, Song H, Liu B Front Immunol. 2023; 14:1114041.

PMID: 37153619 PMC: 10162406. DOI: 10.3389/fimmu.2023.1114041.

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