Regeneration of Dentin-pulp Complex by Using Dental Pulp Stem Cells in Dog

Overview

Journal Dent Res J (Isfahan)

Specialty Dentistry

Date 2021 Nov 11

PMID 34760077

Authors

Fatemeh Dehghani Nazhvani

Setareh Kazempour

Seyed-Mojtaba Hosseini

Ali Dehghani Nazhvani

Pardis Haddadi

Affiliations

Soon will be listed here.

Abstract

Introduction: Although missing tooth is not life-threatening, it affects the quality of daily life. Stem cells have emerged as an important player in the generation and maintenance of many tissues. The role of scaffolds has changed from a passive carrier to a bioactive matrix, which can be used to induce cellular behavior. The aim of this study was to determine the possibility of regeneration of dentin-pulp complex with dental pulp stem cells (DPSCs) in an animal model.

Materials And Methods: In this animal study after extraction of DPSCs and cultivation, 10 types of scaffolds were made by using platelet-rich plasma (PRP), cancellous bone, and collagen pad. They were inserted in different parts of the dog's mouth. After the 4 month, the area was operated, and the scaffolds were removed.

Results: Microscopic examination revealed no sign of cell differentiation and formation of new structures in those models which used collagen scaffolds. However, the dentin-pulp complex emerged in models that the combination of bone scaffolds and PRP or stem cells was used.

Conclusion: Using bone scaffolds in combination with PRP or DPSCs to regenerate dentin-pulp complex in dog helped odontoblastic and pulpal differentiation as well as the formation of predentin and tubular dentin.

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