CryoEM Structure of the Outer Membrane Secretin Channel PIV from the F1 Filamentous Bacteriophage

Overview

Journal Nat Commun

Specialty Biology

Date 2021 Nov 3

PMID 34728631

Citations 12

Authors

Rebecca Conners

Mathew McLaren

Urszula Lapinska

Kelly Sanders

M Rhia L Stone

Mark A T Blaskovich

Stefano Pagliara

Bertram Daum

Jasna Rakonjac

Vicki A M Gold

Affiliations

Soon will be listed here.

Abstract

The Ff family of filamentous bacteriophages infect gram-negative bacteria, but do not cause lysis of their host cell. Instead, new virions are extruded via the phage-encoded pIV protein, which has homology with bacterial secretins. Here, we determine the structure of pIV from the f1 filamentous bacteriophage at 2.7 Å resolution by cryo-electron microscopy, the first near-atomic structure of a phage secretin. Fifteen f1 pIV subunits assemble to form a gated channel in the bacterial outer membrane, with associated soluble domains projecting into the periplasm. We model channel opening and propose a mechanism for phage egress. By single-cell microfluidics experiments, we demonstrate the potential for secretins such as pIV to be used as adjuvants to increase the uptake and efficacy of antibiotics in bacteria. Finally, we compare the f1 pIV structure to its homologues to reveal similarities and differences between phage and bacterial secretins.

Citing Articles

Phage-induced efflux down-regulation boosts antibiotic efficacy.

Kraus S, Fletcher M, Lapinska U, Chawla K, Baker E, Attrill E PLoS Pathog. 2024; 20(6):e1012361.

PMID: 38941361 PMC: 11239113. DOI: 10.1371/journal.ppat.1012361.

Expression, Purification, and Cryo-EM Structural Analysis of an Outer Membrane Secretin Channel.

Conners R, McLaren M, Russel M, Gold V Methods Mol Biol. 2024; 2778:291-310.

PMID: 38478285 DOI: 10.1007/978-1-0716-3734-0_18.

Insights into dynamics and gating properties of T2SS secretins.

Barbat B, Douzi B, Ball G, Tribout M, El Karkouri K, Kellenberger C Sci Adv. 2023; 9(40):eadg6996.

PMID: 37792935 PMC: 10550240. DOI: 10.1126/sciadv.adg6996.

Characterisation of the Novel Filamentous Phage PMBT54 Infecting the Milk Spoilage Bacteria and .

Hille F, Gieschler S, Brinks E, Franz C Viruses. 2023; 15(9).

PMID: 37766190 PMC: 10534721. DOI: 10.3390/v15091781.

Assembly mechanism of a Tad secretion system secretin-pilotin complex.

Tassinari M, Rudzite M, Filloux A, Low H Nat Commun. 2023; 14(1):5643.

PMID: 37704603 PMC: 10499894. DOI: 10.1038/s41467-023-41200-1.

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