Genotypes of Echinococcus Species from Cattle in Tanzania

Overview

Journal Korean J Parasitol

Specialty Parasitology

Date 2021 Nov 2

PMID 34724764

Authors

Mohammed Mebarek Bia

Seongjun Choe

Barakaeli Abdieli Ndosi

Hansol Park

Yeseul Kang

Chatanun Eamudomkarn

Tilak Chandra Nath

Sunmin Kim

Hyeong-Kyu Jeon

Dongmin Lee

Keeseon S Eom

Affiliations

Soon will be listed here.

Abstract

Cystic echinococcosis is a zoonotic parasitic disease caused by Echinococcus species. Tanzania is one of the endemic countries with cystic echinococcosis. This study focussed on identifying genotypes of Echinococcus spp. in Tanzania. We collected 7 cysts from cattle in Mwanza municipal (n=4) and Loliondo district (n=3). The cysts from Mwanza were all E. ortleppi and fertile. In contrast, the cysts from Loliondo were all E. granulosus sensu stricto and sterile. Two from the 4 cysts were a new haplotype of E. ortleppi (G5). These results can improve the preventive and control programs for humans and livestock in Tanzania. To our knowledge, this study is considered the first to identify the genotype and haplotype of Echinococcus spp. in Tanzania.

References

Amer S, Helal I, Kamau E, Feng Y, Xiao L . Molecular characterization of Echinococcus granulosus sensu lato from farm animals in Egypt. PLoS One. 2015; 10(3):e0118509. PMC: 4356597. DOI: 10.1371/journal.pone.0118509. View

Hodzic A, Alic A, Supic J, Skapur V, Duscher G . Echinococcus ortleppi, the cattle strain in a crested porcupine (Hystrix cristata): A new host record. Vet Parasitol. 2018; 256:32-34. DOI: 10.1016/j.vetpar.2018.05.004. View

Jusabani A, Kalambo C, Jusabani M, Surani S . Myocardial Echinococcosis: Rare Manifestation of a Common Parasitic Disease in Northern Tanzania. Cureus. 2020; 12(6):e8681. PMC: 7370672. DOI: 10.7759/cureus.8681. View

Ohiolei J, Li L, Ebhodaghe F, Yan H, Isaac C, Bo X . Prevalence and distribution of Echinococcus spp. in wild and domestic animals across Africa: A systematic review and meta-analysis. Transbound Emerg Dis. 2020; 67(6):2345-2364. DOI: 10.1111/tbed.13571. View

Gordo F, Bandera C . Differentiation of Spanish strains of Echinococcus granulosus using larval rostellar hook morphometry. Int J Parasitol. 1997; 27(1):41-9. DOI: 10.1016/s0020-7519(96)00173-7. View

Laurimae T, Kinkar L, Romig T, Omer R, Casulli A, Umhang G . The benefits of analysing complete mitochondrial genomes: Deep insights into the phylogeny and population structure of Echinococcus granulosus sensu lato genotypes G6 and G7. Infect Genet Evol. 2018; 64:85-94. DOI: 10.1016/j.meegid.2018.06.016. View

Tamura K, Stecher G, Peterson D, Filipski A, Kumar S . MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Mol Biol Evol. 2013; 30(12):2725-9. PMC: 3840312. DOI: 10.1093/molbev/mst197. View

Salamatin R, Kowal J, Nosal P, Kornas S, Cielecka D, Janczak D . Cystic echinococcosis in Poland: genetic variability and the first record of Echinococcus granulosus sensu stricto (G1 genotype) in the country. Parasitol Res. 2017; 116(11):3077-3085. PMC: 5660138. DOI: 10.1007/s00436-017-5618-4. View

Terefe Y, Addy F, Alemu S, Mackenstedt U, Romig T, Wassermann M . Genetic characterization of Echinococcus species in eastern Ethiopia. Vet Parasitol Reg Stud Reports. 2019; 17:100302. DOI: 10.1016/j.vprsr.2019.100302. View

10.

Addy F, Wassermann M, Banda F, Mbaya H, Aschenborn J, Aschenborn O . Genetic polymorphism and population structure of Echinococcus ortleppi. Parasitology. 2016; 144(4):450-458. DOI: 10.1017/S0031182016001840. View

11.

Said I, Abdel-Hafez S . Morphological variation of Echinococcus granulosus protoscoleces from hydatid cysts of human and various domestic animals in Jordan. Int J Parasitol. 1988; 18(8):1111-4. DOI: 10.1016/0020-7519(88)90083-5. View

12.

Nakao M, Yokoyama N, Sako Y, Fukunaga M, Ito A . The complete mitochondrial DNA sequence of the cestode Echinococcus multilocularis (Cyclophyllidea: Taeniidae). Mitochondrion. 2005; 1(6):497-509. DOI: 10.1016/s1567-7249(02)00040-5. View

13.

Rozas J, Ferrer-Mata A, Sanchez-DelBarrio J, Guirao-Rico S, Librado P, Ramos-Onsins S . DnaSP 6: DNA Sequence Polymorphism Analysis of Large Data Sets. Mol Biol Evol. 2017; 34(12):3299-3302. DOI: 10.1093/molbev/msx248. View

14.

Morgan J, Blair D . Relative merits of nuclear ribosomal internal transcribed spacers and mitochondrial CO1 and ND1 genes for distinguishing among Echinostoma species (Trematoda). Parasitology. 1998; 116 ( Pt 3):289-97. DOI: 10.1017/s0031182097002217. View

15.

Mogoye B, Menezes C, Wong M, Stacey S, von Delft D, Wahlers K . First insights into species and genotypes of Echinococcus in South Africa. Vet Parasitol. 2013; 196(3-4):427-32. DOI: 10.1016/j.vetpar.2013.03.033. View

16.

Ohiolei J, Yan H, Li L, Magaji A, Luka J, Zhu G . Cystic echinococcosis in Nigeria: first insight into the genotypes of Echinococcus granulosus in animals. Parasit Vectors. 2019; 12(1):392. PMC: 6686243. DOI: 10.1186/s13071-019-3644-z. View

17.

Felsenstein J . Evolutionary trees from DNA sequences: a maximum likelihood approach. J Mol Evol. 1981; 17(6):368-76. DOI: 10.1007/BF01734359. View

18.

Kinkar L, Laurimae T, Balkaya I, Casulli A, Zait H, Irshadullah M . Genetic diversity and phylogeography of the elusive, but epidemiologically important Echinococcus granulosus sensu stricto genotype G3. Parasitology. 2018; 145(12):1613-1622. DOI: 10.1017/S0031182018000549. View

19.

Ernest E, Nonga H, Kynsieri N, Cleaveland S . A retrospective survey of human hydatidosis based on hospital records during the period 1990-2003 in Ngorongoro, Tanzania. Zoonoses Public Health. 2009; 57(7-8):e124-9. DOI: 10.1111/j.1863-2378.2009.01297.x. View

20.

Ngowi H, Kassuku A, Maeda G, Boa M, Willingham A . A slaughter slab survey for extra-intestinal porcine helminth infections in northern Tanzania. Trop Anim Health Prod. 2004; 36(4):335-40. DOI: 10.1023/b:trop.0000026663.07862.2a. View