Cystic Echinococcosis in Poland: Genetic Variability and the First Record of Echinococcus Granulosus Sensu Stricto (G1 Genotype) in the Country

Overview

Journal Parasitol Res

Specialty Parasitology

Date 2017 Oct 5

PMID 28975403

Citations 7

Authors

Ruslan Salamatin

Jerzy Kowal

Pawel Nosal

Slawomir Kornas

Danuta Cielecka

Dawid Janczak

Waldemar Patkowski

Jakub Gawor

Vadim Kornyushin

Elzbieta Golab

Viliam Snabel

Affiliations

Soon will be listed here.

Abstract

Cystic echinococcosis is one of the most important zoonotic diseases affecting humans and livestock worldwide, and is endemic in Poland. A set of six isolates on larval stages of Echinococcus granulosus sensu lato tapeworms collected from three humans, two pigs and one sheep from Polish foci of CE was examined by DNA sequencing of two mitochondrial genes (cox1, rrnS). The results demonstrated the presence of E. canadensis and E. granulosus sensu stricto in the investigated hydatid cysts. The former species was found in all five isolates from pigs and humans derived from central Poland. In a sheep hydatid cyst originating from Lesser Poland Voivodeship in southern Poland, E. granulosus s. s. (G1 genotype) was identified. This is the first report of an unambiguously autochthonous infection with E. granulosus s. s. in Poland. The global distribution and host affiliations of the commonly occurring G1 microvariant with nucleotide change 56C/T in cox1, detected here in Polish sheep, are discussed. The finding that sheep harboured E. granulosus s. s. may have important consequences for developing effective hydatid control programmes in Poland due to its longer maturation rate in dogs compared with E. canadensis G7. This may lead to greater expenditures for purchasing anthelmintics to provide an appropriate dosing regime in sheep-raising areas of the country.

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References

Gholami S, Jahandar H, Abastabar M, Pagheh A, Mobedi I, Sharbatkhori M . Sensu Stricto in Dogs and Jackals from Caspian Sea Region, Northern Iran. Iran J Parasitol. 2017; 11(2):186-194. PMC: 5236095. View

Thompson R, Kumaratilake L, Eckert J . Observations on Echinococcus granulosus of cattle origin in Switzerland. Int J Parasitol. 1984; 14(3):283-91. DOI: 10.1016/0020-7519(84)90079-1. View

Haag K, Ayala F, Kamenetzky L, Gutierrez A, Rosenzvit M . Livestock trade history, geography, and parasite strains: the mitochondrial genetic structure of Echinococcus granulosus in Argentina. J Parasitol. 2004; 90(2):234-9. DOI: 10.1645/GE-173R. View

Dinkel A, Njoroge E, Zimmermann A, Walz M, Zeyhle E, Elmahdi I . A PCR system for detection of species and genotypes of the Echinococcus granulosus-complex, with reference to the epidemiological situation in eastern Africa. Int J Parasitol. 2004; 34(5):645-53. DOI: 10.1016/j.ijpara.2003.12.013. View

Zait H, Kouidri M, Grenouillet F, Umhang G, Millon L, Hamrioui B . Molecular characterization of Echinococcus granulosus sensu stricto and Echinococcus canadensis in humans and livestock from Algeria. Parasitol Res. 2016; 115(6):2423-31. DOI: 10.1007/s00436-016-4994-5. View

Cardona G, Carmena D . A review of the global prevalence, molecular epidemiology and economics of cystic echinococcosis in production animals. Vet Parasitol. 2012; 192(1-3):10-32. DOI: 10.1016/j.vetpar.2012.09.027. View

Schneider R, Gollackner B, Schindl M, Tucek G, Auer H . Echinococcus canadensis G7 (pig strain): an underestimated cause of cystic echinococcosis in Austria. Am J Trop Med Hyg. 2010; 82(5):871-4. PMC: 2861383. DOI: 10.4269/ajtmh.2010.09-0639. View

Pezeshki A, Akhlaghi L, Sharbatkhori M, Razmjou E, Oormazdi H, Mohebali M . Genotyping of Echinococcus granulosus from domestic animals and humans from Ardabil Province, northwest Iran. J Helminthol. 2012; 87(4):387-91. DOI: 10.1017/S0022149X1200051X. View

Busi M, Snabel V, Varcasia A, Garippa G, Perrone V, De Liberato C . Genetic variation within and between G1 and G3 genotypes of Echinococcus granulosus in Italy revealed by multilocus DNA sequencing. Vet Parasitol. 2007; 150(1-2):75-83. DOI: 10.1016/j.vetpar.2007.09.003. View

10.

Farhadi M, Fazaeli A, Haniloo A . Genetic characterization of livestock and human hydatid cyst isolates from northwest Iran, using the mitochondrial cox1 gene sequence. Parasitol Res. 2015; 114(12):4363-70. DOI: 10.1007/s00436-015-4673-y. View

11.

Boufana B, Stidworthy M, Bell S, Chantrey J, Masters N, Unwin S . Echinococcus and Taenia spp. from captive mammals in the United Kingdom. Vet Parasitol. 2012; 190(1-2):95-103. DOI: 10.1016/j.vetpar.2012.05.023. View

12.

Pour A, Hosseini S, Shayan P . Comparative genotyping of Echinococcus granulosus infecting buffalo in Iran using cox1 gene. Parasitol Res. 2010; 108(5):1229-34. DOI: 10.1007/s00436-010-2170-x. View

13.

Umhang G, Chihai O, Boue F . Molecular characterization of Echinococcus granulosus in a hyperendemic European focus, the Republic of Moldova. Parasitol Res. 2014; 113(12):4371-6. DOI: 10.1007/s00436-014-4112-5. View

14.

Bowles J, Hope M, Tiu W, Liu X, McManus D . Nuclear and mitochondrial genetic markers highly conserved between Chinese and Philippine Schistosoma japonicum. Acta Trop. 1993; 55(4):217-29. DOI: 10.1016/0001-706x(93)90079-q. View

15.

Dybicz M, Gierczak A, Dabrowska J, Rdzanek L, Michalowicz B . Molecular diagnosis of cystic echinococcosis in humans from central Poland. Parasitol Int. 2013; 62(4):364-7. DOI: 10.1016/j.parint.2013.03.005. View

16.

Laurimae T, Kinkar L, Andresiuk V, Haag K, Ponce-Gordo F, Acosta-Jamett G . Genetic diversity and phylogeography of highly zoonotic Echinococcus granulosus genotype G1 in the Americas (Argentina, Brazil, Chile and Mexico) based on 8279bp of mtDNA. Infect Genet Evol. 2016; 45:290-296. DOI: 10.1016/j.meegid.2016.09.015. View

17.

Konyaev S, Yanagida T, Ivanov M, Ruppel V, Sako Y, Nakao M . The first report on cystic echinococcosis in a cat caused by Echinococcus granulosus sensu stricto (G1). J Helminthol. 2011; 86(4):391-4. DOI: 10.1017/S0022149X1100054X. View

18.

Kumaratilake L, Thompson R, DUNSMORE J . Comparative strobilar development of Echinococcus granulosus of sheep origin from different geographical areas of Australia in vivo and in vitro. Int J Parasitol. 1983; 13(2):151-6. DOI: 10.1016/0020-7519(83)90005-x. View

19.

Mrad S, Filisetti D, Oudni M, Mekki M, Belguith M, Nouri A . Molecular evidence of ovine (G1) and camel (G6) strains of Echinococcus granulosus in Tunisia and putative role of cattle in human contamination. Vet Parasitol. 2005; 129(3-4):267-72. DOI: 10.1016/j.vetpar.2005.02.006. View

20.

Snabel V, DAmelio S, Nakao M, Romig T, Yolasigmaz A, Gunes K . Cystic echinococcosis in Turkey: genetic variability and first record of the pig strain (G7) in the country. Parasitol Res. 2009; 105(1):145-54. DOI: 10.1007/s00436-009-1376-2. View