Hybrid Antibody-Aptamer Assay for Detection of Tetrodotoxin in Pufferfish

Overview

Journal Anal Chem

Specialty Chemistry

Date 2021 Oct 26

PMID 34697940

Citations 14

Authors

Xhensila Shkembi

Vasso Skouridou

Marketa Svobodova

Sandra Leonardo

Abdulaziz S Bashammakh

Abdulrahman O Alyoubi

Monica Campas

Ciara K O Sullivan

Affiliations

Soon will be listed here.

Abstract

The marine toxin tetrodotoxin (TTX) poses a great risk to public health safety due to its severe paralytic effects after ingestion. Seafood poisoning caused by the consumption of contaminated marine species like pufferfish due to its expansion to nonendemic areas has increased the need for fast and reliable detection of the toxin to effectively implement prevention strategies. Liquid chromatography-mass spectrometry is considered the most accurate method, although competitive immunoassays have also been reported. In this work, we sought to develop an aptamer-based assay for the rapid, sensitive, and cost-effective detection of TTX in pufferfish. Using capture-SELEX combined with next-generation sequencing, aptamers were identified, and their binding properties were evaluated. Finally, a highly sensitive and user-friendly hybrid antibody-aptamer sandwich assay was developed with superior performance compared to several assays reported in the literature and commercial immunoassay kits. The assay was successfully applied to the quantification of TTX in pufferfish extracts, and the results obtained correlated very well with a competitive magnetic bead-based immunoassay performed in parallel for comparison. This is one of the very few works reported in the literature of such hybrid assays for small-molecule analytes whose compatibility with field samples is also demonstrated.

Citing Articles

Tetrodotoxin: The State-of-the-Art Progress in Characterization, Detection, Biosynthesis, and Transport Enrichment.

Zhang X, Qiao K, Cui R, Xu M, Cai S, Huang Q Mar Drugs. 2024; 22(12).

PMID: 39728106 PMC: 11676112. DOI: 10.3390/md22120531.

Preliminary Development of DNA Aptamer Quantum Dot-Based Competitive Lateral Flow Assays for Saxitoxin and Tetrodotoxin.

Bruno J J Fluoresc. 2024; .

PMID: 39578342 DOI: 10.1007/s10895-024-04049-1.

Tetrodotoxin and the state-of-the-art progress of its associated analytical methods.

Mi W, Liu S Front Microbiol. 2024; 15:1413741.

PMID: 39290516 PMC: 11407752. DOI: 10.3389/fmicb.2024.1413741.

Recent research progress in tetrodotoxin detection and quantitative analysis methods.

Lin C, Li Q, Liu D, Feng Q, Zhou H, Shi B Front Chem. 2024; 12:1447312.

PMID: 39206441 PMC: 11349515. DOI: 10.3389/fchem.2024.1447312.

Automated Patch Clamp for the Detection of Tetrodotoxin in Pufferfish Samples.

Campas M, Reverte J, Tudo A, Alkassar M, Diogene J, Sureda F Mar Drugs. 2024; 22(4).

PMID: 38667793 PMC: 11050952. DOI: 10.3390/md22040176.

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