A SNP Mutation in Homeodomain-DDT (HD-DDT) Transcription Factor Results in () in Cucumber ( L.)

Overview

Journal Genes (Basel)

Publisher MDPI

Date 2021 Oct 23

PMID 34680876

Citations 2

Authors

Zhige Yang

Mengfei Song

Feng Cheng

Mengru Zhang

Marzieh Davoudi

Jinfeng Chen

Qunfeng Lou

Affiliations

Soon will be listed here.

Abstract

Trichome is a natural physical barrier protecting plants against environmental stresses, natural infestations, ultraviolet rays and pathogenicity. Trichome also helps plants in maintaining appropriate water content by reducing transpiration rate. The molecular mechanism regulating unicellular trichome development in Arabidopsis has been extensively elucidated, but the molecular mechanism regulating multicellular trichome development remains unclear. In this study, we identified a () mutant from a cucumber EMS (Ethylmethylsulfone) mutagenesis population. Genetic analysis indicated that an incomplete dominant gene controls the trait. Using a combination of map-based cloning and BSA-seq (Bulked Segregant Analysis -Sequencing), we identified the candidate gene, , responsible for the mutation. Sequence alignment revealed one base substitution in gene , resulting in an amino acid substitution. The deduced amino acid sequence of encodes a HD-DDT (homeodomain-DDT) transcriptional regulatory protein containing a conserved homeobox domain and a DDT domain. Gene expression analysis revealed that the expression level of in the mutant was similar to that in the WT (wild type). Transcriptome analysis indicated that the gene may regulate the development of the epidermis by influencing plant hormone signaling pathways or participating in several transcription factor pathways. The results of this study are fundamental for a better understanding of the function of the HD-DDT transcription factor in the trichome development of cucumber.

Citing Articles

Localization of potato browning resistance genes based on BSA-seq technology.

Wang H, Pang Z, Wang L, Tian G, Li F, Pan Y PeerJ. 2024; 12:e17831.

PMID: 39131626 PMC: 11313402. DOI: 10.7717/peerj.17831.

Molecular Mechanisms of Plant Trichome Development.

Han G, Li Y, Yang Z, Wang C, Zhang Y, Wang B Front Plant Sci. 2022; 13:910228.

PMID: 35720574 PMC: 9198495. DOI: 10.3389/fpls.2022.910228.

References

Liu X, Wang T, Bartholomew E, Black K, Dong M, Zhang Y . Comprehensive analysis of NAC transcription factors and their expression during fruit spine development in cucumber ( L.). Hortic Res. 2018; 5:31. PMC: 5981648. DOI: 10.1038/s41438-018-0036-z. View

Sato Y, Hong S, Tagiri A, Kitano H, Yamamoto N, Nagato Y . A rice homeobox gene, OSH1, is expressed before organ differentiation in a specific region during early embryogenesis. Proc Natl Acad Sci U S A. 1996; 93(15):8117-22. PMC: 38885. DOI: 10.1073/pnas.93.15.8117. View

Chen C, Liu M, Jiang L, Liu X, Zhao J, Yan S . Transcriptome profiling reveals roles of meristem regulators and polarity genes during fruit trichome development in cucumber (Cucumis sativus L.). J Exp Bot. 2014; 65(17):4943-58. PMC: 4144775. DOI: 10.1093/jxb/eru258. View

Jouannic S, Collin M, Vidal B, Verdeil J, Tregear J . A class I KNOX gene from the palm species Elaeis guineensis (Arecaceae) is associated with meristem function and a distinct mode of leaf dissection. New Phytol. 2007; 174(3):551-568. DOI: 10.1111/j.1469-8137.2007.02020.x. View

Kazama H, Dan H, Imaseki H, Wasteneys G . Transient exposure to ethylene stimulates cell division and alters the fate and polarity of hypocotyl epidermal cells. Plant Physiol. 2004; 134(4):1614-23. PMC: 419835. DOI: 10.1104/pp.103.031088. View

Saitou N, Nei M . The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol. 1987; 4(4):406-25. DOI: 10.1093/oxfordjournals.molbev.a040454. View

Walker A, Davison P, James C, Srinivasan N, Blundell T, Esch J . The TRANSPARENT TESTA GLABRA1 locus, which regulates trichome differentiation and anthocyanin biosynthesis in Arabidopsis, encodes a WD40 repeat protein. Plant Cell. 1999; 11(7):1337-50. PMC: 144274. DOI: 10.1105/tpc.11.7.1337. View

Bryant L, Patole C, Cramer R . Proteomic analysis of the medicinal plant Artemisia annua: Data from leaf and trichome extracts. Data Brief. 2016; 7:325-31. PMC: 4781977. DOI: 10.1016/j.dib.2016.02.038. View

Chang J, Yu T, Yang Q, Li C, Xiong C, Gao S . Hair, encoding a single C2H2 zinc-finger protein, regulates multicellular trichome formation in tomato. Plant J. 2018; 96(1):90-102. DOI: 10.1111/tpj.14018. View

10.

Schiefelbein J . Cell-fate specification in the epidermis: a common patterning mechanism in the root and shoot. Curr Opin Plant Biol. 2002; 6(1):74-8. DOI: 10.1016/s136952660200002x. View

11.

Pan J, Zhang L, Chen G, Wen H, Chen Y, Du H . Study of micro-trichome (mict) reveals novel connections between transcriptional regulation of multicellular trichome development and specific metabolism in cucumber. Hortic Res. 2021; 8(1):21. PMC: 7848009. DOI: 10.1038/s41438-020-00456-0. View

12.

Tian H, Wang X, Guo H, Cheng Y, Hou C, Chen J . NTL8 Regulates Trichome Formation in Arabidopsis by Directly Activating R3 MYB Genes and . Plant Physiol. 2017; 174(4):2363-2375. PMC: 5543959. DOI: 10.1104/pp.17.00510. View

13.

Zhou Z, Sun L, Zhao Y, An L, Yan A, Meng X . Zinc Finger Protein 6 (ZFP6) regulates trichome initiation by integrating gibberellin and cytokinin signaling in Arabidopsis thaliana. New Phytol. 2013; 198(3):699-708. DOI: 10.1111/nph.12211. View

14.

Castellano M, Sablowski R . Intercellular signalling in the transition from stem cells to organogenesis in meristems. Curr Opin Plant Biol. 2005; 8(1):26-31. DOI: 10.1016/j.pbi.2004.11.010. View

15.

Yang C, Li H, Zhang J, Luo Z, Gong P, Zhang C . A regulatory gene induces trichome formation and embryo lethality in tomato. Proc Natl Acad Sci U S A. 2011; 108(29):11836-41. PMC: 3141934. DOI: 10.1073/pnas.1100532108. View

16.

Oppenheimer D, Herman P, Sivakumaran S, Esch J, Marks M . A myb gene required for leaf trichome differentiation in Arabidopsis is expressed in stipules. Cell. 1991; 67(3):483-93. DOI: 10.1016/0092-8674(91)90523-2. View

17.

Wagner G, Wang E, Shepherd R . New approaches for studying and exploiting an old protuberance, the plant trichome. Ann Bot. 2003; 93(1):3-11. PMC: 4242265. DOI: 10.1093/aob/mch011. View

18.

Zhu H, Sun X, Zhang Q, Song P, Hu Q, Zhang X . GLABROUS (CmGL) encodes a HD-ZIP IV transcription factor playing roles in multicellular trichome initiation in melon. Theor Appl Genet. 2017; 131(3):569-579. DOI: 10.1007/s00122-017-3019-9. View

19.

Zhang L, Lv D, Pan J, Zhang K, Wen H, Chen Y . A SNP of HD-ZIP I transcription factor leads to distortion of trichome morphology in cucumber (Cucumis sativus L.). BMC Plant Biol. 2021; 21(1):182. PMC: 8052656. DOI: 10.1186/s12870-021-02955-1. View

20.

Traw M, Bergelson J . Interactive effects of jasmonic acid, salicylic acid, and gibberellin on induction of trichomes in Arabidopsis. Plant Physiol. 2003; 133(3):1367-75. PMC: 281631. DOI: 10.1104/pp.103.027086. View