MicroRNA-218 Aggravates HO-induced Damage in PC12 Cells Via Spred2-mediated Autophagy

Overview

Journal Exp Ther Med

Specialty Pathology

Date 2021 Oct 18

PMID 34659498

Authors

Duoping Chen

Chunmei Li

Rui Lv

Affiliations

Soon will be listed here.

Abstract

The current study aimed to investigate the effects and underlying mechanism of miR-218 in HO-induced neuronal injury. The impacts of miR-218 knockdown on cell viability, apoptosis and autophagy-associated proteins were detected by Cell Counting Kit-8 assay, flow cytometry and western blotting in HO-injured PC12 cells, respectively. Reverse transcription-quantitative PCR (RT-qPCR) and western blotting was executed to explore the expression level of miR-218 and sprouty-related EVH1 domainprotein2 (spred2) in HO-stimulated cells. Besides, the regulatory association between miR-218 and spred2 was explored through bioinformatics and luciferase reporter assay. Following knockdown of miR-218 and spred2, the functions of miR-218 and spred2 in HO-injured cells were further studied. High expression level of miR-218 was observed in HO-disposed PC12 cells, while spred2 expression level was downregulated. Knockdown of miR-218 expression alleviated HO-induced PC12 cell injury by increasing cell proliferation, and decreasing apoptosis and autophagy. Furthermore, spred2 was identified as a direct target of miR-218 and was negatively regulated by miR-218. Moreover, suppression of spred2 abrogated the protective effects of miR-218 inhibition on HO-injured PC12 cells. Depletion of miR-218 protected PC12 cells against HO-induced cell injury via the upregulation of spred2, which provided a promising therapeutic strategy for spinal cord injury.

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