MiR-130a-3p Suppresses Colorectal Cancer Growth by Targeting Wnt Family Member 1 (WNT1)

Overview

Journal Bioengineered

Date 2021 Oct 18

PMID 34657551

Citations 12

Authors

Guang-Lin Song

Ming Xiao

Xiao-Ya Wan

Jun Deng

Jun-Da Ling

Ying-Guo Tian

Min Li

Jie Yin

Ren-Ying Zheng

Yi Tang

Gui-Yuan Liu

Affiliations

Soon will be listed here.

Abstract

The microRNA miR-130a-3p (miR-130a-3p) has anti-tumor activity against numerous cancer types. Further, miR-130a-3p may target Wnt signaling, which is a critical pathway regulating tumorigenesis. Functions of miR-130a-3p in colorectal cancer (CRC) and contributions of Wnt1 pathway modulation, however, have not been examined, hence the exploration on these two aspects. In this study, in comparison with normal controls, both CRC tissue and multiple CRC cell lines showed downregulated miR-130a-3p. MiR-130a-3p overexpression contributed to a decrease in CRC cell proliferation. Additionally, its overexpression also caused reduced expression of WNT Family Member 1 (WNT1) and downstream WNT pathway factors c-myc and cyclin D1. Dual-luciferase assay revealed WNT1 as a direct target of miR-130a-3p, and further the inhibitory effect of miR-130a-3p on c-myc and cyclin D1 was proved to be reversed by overexpressed WNT1. Collectively, miR-130a-3p inhibits CRC growth by directly targeting WNT1, and miR-130a-3p and WNT1 pathway-associated factors are defined as potential targets for CRC treatment.

Citing Articles

Wnt/β-catenin signaling pathway: proteins' roles in osteoporosis and cancer diseases and the regulatory effects of natural compounds on osteoporosis.

Wang X, Qu Z, Zhao S, Luo L, Yan L Mol Med. 2024; 30(1):193.

PMID: 39468464 PMC: 11520425. DOI: 10.1186/s10020-024-00957-x.

The expression and biological role of complement C1s in esophageal squamous cell carcinoma.

Ge R, Luan Z, Guo T, Xia S, Ye J, Xu J Open Life Sci. 2024; 19(1):20220915.

PMID: 39071493 PMC: 11282917. DOI: 10.1515/biol-2022-0915.

Exploring the Key Signaling Pathways and ncRNAs in Colorectal Cancer.

Lee Y, Kim W, Park E, Lee D, Kim J, Shin H Int J Mol Sci. 2024; 25(8).

PMID: 38674135 PMC: 11050203. DOI: 10.3390/ijms25084548.

KLF3 promotes colorectal cancer growth by activating WNT1.

Shen W, Yuan L, Hao B, Xiang J, Cheng F, Wu Z Aging (Albany NY). 2024; 16(3):2475-2493.

PMID: 38305787 PMC: 10911342. DOI: 10.18632/aging.205494.

Analyzing of colorectal cancerrelated genes and microRNAs expression profiles in response to probiotics Lactobacillus acidophilus and Saccharomyces cerevisiae in colon cancer cell lines.

Saffar K, Larypoor M, Torbati M Mol Biol Rep. 2024; 51(1):122.

PMID: 38227272 DOI: 10.1007/s11033-023-09008-w.

References

Wang M, Wang X, Liu W . MicroRNA‑130a‑3p promotes the proliferation and inhibits the apoptosis of cervical cancer cells via negative regulation of RUNX3. Mol Med Rep. 2020; 22(4):2990-3000. DOI: 10.3892/mmr.2020.11368. View

Clevers H . Wnt/beta-catenin signaling in development and disease. Cell. 2006; 127(3):469-80. DOI: 10.1016/j.cell.2006.10.018. View

Chen W, Zhang Y, Li Y, Zhang J, Zhang T, Fu B . Constitutive expression of Wnt/β‑catenin target genes promotes proliferation and invasion of liver cancer stem cells. Mol Med Rep. 2016; 13(4):3466-74. PMC: 4805073. DOI: 10.3892/mmr.2016.4986. View

Hatley M, Patrick D, Garcia M, Richardson J, Bassel-Duby R, Van Rooij E . Modulation of K-Ras-dependent lung tumorigenesis by MicroRNA-21. Cancer Cell. 2010; 18(3):282-93. PMC: 2971666. DOI: 10.1016/j.ccr.2010.08.013. View

Takamizawa J, Konishi H, Yanagisawa K, Tomida S, Osada H, Endoh H . Reduced expression of the let-7 microRNAs in human lung cancers in association with shortened postoperative survival. Cancer Res. 2004; 64(11):3753-6. DOI: 10.1158/0008-5472.CAN-04-0637. View

Tian X, Fei Q, Du M, Zhu H, Ye J, Qian L . miR-130a-3p regulated TGF-β1-induced epithelial-mesenchymal transition depends on SMAD4 in EC-1 cells. Cancer Med. 2019; 8(3):1197-1208. PMC: 6434193. DOI: 10.1002/cam4.1981. View

Bartel D . MicroRNAs: target recognition and regulatory functions. Cell. 2009; 136(2):215-33. PMC: 3794896. DOI: 10.1016/j.cell.2009.01.002. View

OConnell J, Maggard M, Ko C . Colon cancer survival rates with the new American Joint Committee on Cancer sixth edition staging. J Natl Cancer Inst. 2004; 96(19):1420-5. DOI: 10.1093/jnci/djh275. View

Ai L, Luo X, Yan X, Jiang S . MicroRNA-506-3p inhibits colorectal cancer cell proliferation through targeting enhancer of zeste homologue 2. Bioengineered. 2021; 12(1):4044-4053. PMC: 8806550. DOI: 10.1080/21655979.2021.1951930. View

10.

Kara M, Yumrutas O, Ozcan O, Celik O, Bozgeyik E, Bozgeyik I . Differential expressions of cancer-associated genes and their regulatory miRNAs in colorectal carcinoma. Gene. 2015; 567(1):81-6. DOI: 10.1016/j.gene.2015.04.065. View

11.

Mansoori B, Mohammadi A, Naghizadeh S, Gjerstorff M, Shanehbandi D, Shirjang S . miR-330 suppresses EMT and induces apoptosis by downregulating HMGA2 in human colorectal cancer. J Cell Physiol. 2019; 235(2):920-931. DOI: 10.1002/jcp.29007. View

12.

Li Z, Cao Y, Jie Z, Liu Y, Li Y, Li J . miR-495 and miR-551a inhibit the migration and invasion of human gastric cancer cells by directly interacting with PRL-3. Cancer Lett. 2012; 323(1):41-47. DOI: 10.1016/j.canlet.2012.03.029. View

13.

Kong X, Zhang J, Li J, Shao J, Fang L . MiR-130a-3p inhibits migration and invasion by regulating RAB5B in human breast cancer stem cell-like cells. Biochem Biophys Res Commun. 2018; 501(2):486-493. DOI: 10.1016/j.bbrc.2018.05.018. View

14.

Karimi L, Zeinali T, Hosseinahli N, Mansoori B, Mohammadi A, Yousefi M . miRNA-143 replacement therapy harnesses the proliferation and migration of colorectal cancer cells in vitro. J Cell Physiol. 2019; 234(11):21359-21368. DOI: 10.1002/jcp.28745. View

15.

Poodineh J, Sirati-Sabet M, Rajabibazl M, Mohammadi-Yeganeh S . MiR-130a-3p blocks Wnt signaling cascade in the triple-negative breast cancer by targeting the key players at multiple points. Heliyon. 2020; 6(11):e05434. PMC: 7662874. DOI: 10.1016/j.heliyon.2020.e05434. View

16.

Ouyang M, Li Y, Ye S, Ma J, Lu L, Lv W . MicroRNA profiling implies new markers of chemoresistance of triple-negative breast cancer. PLoS One. 2014; 9(5):e96228. PMC: 4008525. DOI: 10.1371/journal.pone.0096228. View

17.

Iida M, Hazama S, Tsunedomi R, Tanaka H, Takenouchi H, Kanekiyo S . Overexpression of miR‑221 and miR‑222 in the cancer stroma is associated with malignant potential in colorectal cancer. Oncol Rep. 2018; 40(3):1621-1631. DOI: 10.3892/or.2018.6575. View

18.

Suresh S, McCallum L, Lu W, Lazar N, Perbal B, Irvine A . MicroRNAs 130a/b are regulated by BCR-ABL and downregulate expression of CCN3 in CML. J Cell Commun Signal. 2011; 5(3):183-91. PMC: 3145871. DOI: 10.1007/s12079-011-0139-x. View

19.

Chen X, Yue B, Zhang C, Qi M, Qiu J, Wang Y . inhibits the viability, proliferation, invasion, and cell cycle, and promotes apoptosis of nasopharyngeal carcinoma cells by suppressing BACH2 expression. Biosci Rep. 2017; 37(3). PMC: 5463266. DOI: 10.1042/BSR20160576. View

20.

Asukai K, Kawamoto K, Eguchi H, Konno M, Asai A, Iwagami Y . Micro-RNA-130a-3p Regulates Gemcitabine Resistance via PPARG in Cholangiocarcinoma. Ann Surg Oncol. 2017; 24(8):2344-2352. DOI: 10.1245/s10434-017-5871-x. View