E. Coli Aggregation and Impaired Cell Division After Terahertz Irradiation

Overview

Journal Sci Rep

Specialty Science

Date 2021 Oct 15

PMID 34650158

Citations 6

Authors

Sergey Peltek

Irina Meshcheryakova

Elena Kiseleva

Dmitry Oshchepkov

Alexei Rozanov

Danil Serdyukov

Evgeniy Demidov

Gennady Vasiliev

Nikolay Vinokurov

Alla Bryanskaya

Svetlana Bannikova

Vasiliy Popik

Tatyana Goryachkovskaya

Affiliations

Soon will be listed here.

Abstract

In this study we demonstrated that exposure of Escherichia coli (E. coli) to terahertz (THz) radiation resulted in a change in the activities of the tdcABCDEFGR and matA-F genes (signs of cell aggregation), gene yjjQ (signs of suppression of cell motility), dicABCF, FtsZ, and minCDE genes (signs of suppression of cell division), sfmACDHF genes (signs of adhesin synthesis), yjbEFGH and gfcA genes (signs of cell envelope stabilization). Moreover, THz radiation induced E. coli csg operon genes of amyloid biosynthesis. Electron microscopy revealed that the irradiated bacteria underwent increased aggregation; 20% of them formed bundle-like structures consisting of two to four pili clumped together. This could be the result of changes in the adhesive properties of the pili. We also found aberrations in cell wall structure in the middle part of the bacterial cell; these aberrations impaired the cell at the initial stages of division and resulted in accumulation of long rod-like cells. Overall, THz radiation was shown to have adverse effects on bacterial populations resulting in cells with abnormal morphology.

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References

Johnson J, Lackner L, de Boer P . Targeting of (D)MinC/MinD and (D)MinC/DicB complexes to septal rings in Escherichia coli suggests a multistep mechanism for MinC-mediated destruction of nascent FtsZ rings. J Bacteriol. 2002; 184(11):2951-62. PMC: 135045. DOI: 10.1128/JB.184.11.2951-2962.2002. View

Anderson D, Gueiros-Filho F, Erickson H . Assembly dynamics of FtsZ rings in Bacillus subtilis and Escherichia coli and effects of FtsZ-regulating proteins. J Bacteriol. 2004; 186(17):5775-81. PMC: 516820. DOI: 10.1128/JB.186.17.5775-5781.2004. View

Shen B, Lutkenhaus J . The conserved C-terminal tail of FtsZ is required for the septal localization and division inhibitory activity of MinC(C)/MinD. Mol Microbiol. 2009; 72(2):410-24. PMC: 2759774. DOI: 10.1111/j.1365-2958.2009.06651.x. View

Chingcuanco F, Yu Y, Kus J, Que L, Lackraj T, Levesque C . Identification of a novel adhesin involved in acid-induced adhesion of enterohaemorrhagic Escherichia coli O157 : H7. Microbiology (Reading). 2012; 158(Pt 9):2399-2407. DOI: 10.1099/mic.0.056374-0. View

Wadhams G, Armitage J . Making sense of it all: bacterial chemotaxis. Nat Rev Mol Cell Biol. 2004; 5(12):1024-37. DOI: 10.1038/nrm1524. View

Cam K, Bejar S, Gil D, Bouche J . Identification and sequence of gene dicB: translation of the division inhibitor from an in-phase internal start. Nucleic Acids Res. 1988; 16(14A):6327-38. PMC: 338298. DOI: 10.1093/nar/16.14.6327. View

Stratmann T, Madhusudan S, Schnetz K . Regulation of the yjjQ-bglJ operon, encoding LuxR-type transcription factors, and the divergent yjjP gene by H-NS and LeuO. J Bacteriol. 2007; 190(3):926-35. PMC: 2223560. DOI: 10.1128/JB.01447-07. View

Danese P, Pratt L, Kolter R . Exopolysaccharide production is required for development of Escherichia coli K-12 biofilm architecture. J Bacteriol. 2000; 182(12):3593-6. PMC: 101973. DOI: 10.1128/JB.182.12.3593-3596.2000. View

Anders S, Pyl P, Huber W . HTSeq--a Python framework to work with high-throughput sequencing data. Bioinformatics. 2014; 31(2):166-9. PMC: 4287950. DOI: 10.1093/bioinformatics/btu638. View

10.

Weightman P . Prospects for the study of biological systems with high power sources of terahertz radiation. Phys Biol. 2012; 9(5):053001. DOI: 10.1088/1478-3975/9/5/053001. View

11.

Szwedziak P, Wang Q, Bharat T, Tsim M, Lowe J . Architecture of the ring formed by the tubulin homologue FtsZ in bacterial cell division. Elife. 2014; 3:e04601. PMC: 4383033. DOI: 10.7554/eLife.04601. View

12.

Sergeeva S, Demidova E, Sinitsyna O, Goryachkovskaya T, Bryanskaya A, Semenov A . 2.3THz radiation: Absence of genotoxicity/mutagenicity in Escherichia coli and Salmonella typhimurium. Mutat Res Genet Toxicol Environ Mutagen. 2016; 803-804:34-8. DOI: 10.1016/j.mrgentox.2016.05.005. View

13.

Demidova E, Goryachkovskaya T, Malup T, Bannikova S, Semenov A, Vinokurov N . Studying the non-thermal effects of terahertz radiation on E. coli/pKatG-GFP biosensor cells. Bioelectromagnetics. 2012; 34(1):15-21. DOI: 10.1002/bem.21736. View

14.

Han H, Kim I, Yun E, Lee J, Cho Y, Jin Y . Overproduction of Exopolysaccharide Colanic Acid by Escherichia coli by Strain Engineering and Media Optimization. Appl Biochem Biotechnol. 2020; 193(1):111-127. DOI: 10.1007/s12010-020-03409-4. View

15.

Serdyukov D, Goryachkovskaya T, Mescheryakova I, Bannikova S, Kuznetsov S, Cherkasova O . Study on the effects of terahertz radiation on gene networks of by means of fluorescent biosensors. Biomed Opt Express. 2020; 11(9):5258-5273. PMC: 7510871. DOI: 10.1364/BOE.400432. View

16.

van Teeffelen S, Wang S, Furchtgott L, Huang K, Wingreen N, Shaevitz J . The bacterial actin MreB rotates, and rotation depends on cell-wall assembly. Proc Natl Acad Sci U S A. 2011; 108(38):15822-7. PMC: 3179079. DOI: 10.1073/pnas.1108999108. View

17.

Bolger A, Lohse M, Usadel B . Trimmomatic: a flexible trimmer for Illumina sequence data. Bioinformatics. 2014; 30(15):2114-20. PMC: 4103590. DOI: 10.1093/bioinformatics/btu170. View

18.

Love M, Huber W, Anders S . Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2. Genome Biol. 2014; 15(12):550. PMC: 4302049. DOI: 10.1186/s13059-014-0550-8. View

19.

Larsonneur F, Martin F, Mallet A, Martinez-Gil M, Semetey V, Ghigo J . Functional analysis of Escherichia coli Yad fimbriae reveals their potential role in environmental persistence. Environ Microbiol. 2016; 18(12):5228-5248. DOI: 10.1111/1462-2920.13559. View

20.

Hirota Y, RYTER A, Jacob F . Thermosensitive mutants of E. coli affected in the processes of DNA synthesis and cellular division. Cold Spring Harb Symp Quant Biol. 1968; 33:677-93. DOI: 10.1101/sqb.1968.033.01.077. View