Study on the Effects of Terahertz Radiation on Gene Networks of by Means of Fluorescent Biosensors

Overview

Journal Biomed Opt Express

Specialty Radiology

Date 2020 Oct 5

PMID 33014613

Citations 9

Authors

Danil S Serdyukov

Tatiana N Goryachkovskaya

Irina A Mescheryakova

Svetlana V Bannikova

Sergei A Kuznetsov

Olga P Cherkasova

Vasiliy M Popik

Sergey E Peltek

Affiliations

Soon will be listed here.

Abstract

Three novel fluorescent biosensors sensitive to terahertz (THz) radiation were developed via transformation of () cells with plasmids, in which a promotor of genes , , or controls the expression of a fluorescent protein. The biosensors were exposed to THz radiation from two sources: a high-intensity pulsed short-wave free electron laser and a low-intensity continuous long-wave IMPATT-diode-based device. The threshold and dynamics of fluorescence were found to depend on radiation parameters and exposure time. Heat shock or chemical stress yielded the absence of fluorescence induction. The biosensors are evaluated to be suitable for studying influence of THz radiation on the activity of gene networks related with considered gene promoters.

Citing Articles

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Nonthermal acceleration of protein hydration by sub-terahertz irradiation.

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Specific Features of the Proteomic Response of Thermophilic Bacterium to Terahertz Irradiation.

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