Ionophore Application for Artificial Oocyte Activation and Its Potential Effect on Morphokinetics: a Sibling Oocyte Study

Overview

Journal J Assist Reprod Genet

Publisher Springer

Specialties Genetics
Reproductive Medicine

Date 2021 Oct 13

PMID 34642877

Citations 7

Authors

Omar Shebl

Philip Sebastian Trautner

Sabine Enengl

Elisabeth Reiter

Christina Allerstorfer

Tamara Rechberger

Peter Oppelt

Thomas Ebner

Affiliations

Soon will be listed here.

Abstract

Purpose: To evaluate whether ionophore application at the oocyte stage changes the morphokinetics of the associated embryos in cases of artificial oocyte activation.

Methods: In a prospective sibling oocyte approach, 78 ICSI patients with suspected fertilization problems had half of their MII-oocytes treated with a ready-to-use ionophore (calcimycin) immediately following ICSI (study group). Untreated ICSI eggs served as the control group. Primary analyses focused on morphokinetic behavior and the presence of irregular cleavages. The rates of fertilization, utilization, pregnancy, and live birth rate were also evaluated.

Results: Ionophore-treated oocytes showed a significantly earlier formation of pronuclei (t2PNa) and a better synchronized third cell cycle (s3) (P < .05). The rate of irregular cleavage was unaffected (P > .05). Ionophore treatment significantly improved the overall rates of fertilization (P < .01) and blastocyst utilization (P < .05).

Conclusion: Ionophore application does not negatively affect cleavage timing nor is it associated with irregular cleavage.

Citing Articles

Oocyte activation for women following intracytoplasmic sperm injection (ICSI).

Kamath M, Vogiatzi P, Sunkara S, Woodward B Cochrane Database Syst Rev. 2024; 12:CD014040.

PMID: 39704318 PMC: 11660229. DOI: 10.1002/14651858.CD014040.pub2.

Early rescue oocyte activation at 5 h post-ICSI is a useful strategy for avoiding unexpected fertilization failure and low fertilization in ICSI cycles.

Xue L, Wang S, Wei P, Liu H, Mao X, Qin J Front Endocrinol (Lausanne). 2024; 14:1301505.

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Good practice recommendations on add-ons in reproductive medicine†.

Lundin K, Bentzen J, Bozdag G, Ebner T, Harper J, Le Clef N Hum Reprod. 2023; 38(11):2062-2104.

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Artificial oocyte activation with Ca ionophore improves reproductive outcomes in patients with fertilization failure and poor embryo development in previous ICSI cycles.

Ruan J, Liang S, Pan J, Chen Z, Teng X Front Endocrinol (Lausanne). 2023; 14:1244507.

PMID: 37635975 PMC: 10455927. DOI: 10.3389/fendo.2023.1244507.

Significant differences in efficiency between two commonly used ionophore solutions for assisted oocyte activation (AOA): a prospective comparison of ionomycin and A23187.

Quintana-Vehi A, Martinez M, Zamora M, Rodriguez A, Vassena R, Miguel-Escalada I J Assist Reprod Genet. 2023; 40(7):1661-1668.

PMID: 37247099 PMC: 10352473. DOI: 10.1007/s10815-023-02833-9.

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