Cell Cycle Arrest and Anti-cancer Potential of Probiotic Against HT-29 Cancer Cells

Overview

Journal Bioimpacts

Specialty Biomedical Engineering

Date 2021 Oct 11

PMID 34631486

Citations 23

Authors

Najme Dehghani

Farzaneh Tafvizi

Parvaneh Jafari

Affiliations

Soon will be listed here.

Abstract

Nowadays, probiotic bacteria have been considered as a factor in the prevention and treatment of cancer, especially by induction of apoptosis. This study aimed to evaluate the cytotoxic, anti-proliferative, and apoptotic effects of the supernatant of probiotic on HT-29 cell line. : Molecular identification of probiotic was carried out using specific primers of 16S rRNA gene and sequencing. HT-29 cells were treated with different concentrations of bacterial supernatants at 24, 48, and 72 hours. MTT assay, Annexin V-FITC, real-time PCR, cell cycle analysis, and DAPI staining tests were conducted to evaluate the induction of apoptosis. The level of cyclin D1 protein was measured by immunocytochemistry method. The supernatant of inhibited the growth of HT-29 cancer cells in a dose- and time-dependent manner. The results of flow cytometry confirmed apoptotic cell death. Probiotic bacterial supernatant caused up-regulation of pro-apoptotic genes including caspase-3, caspase-9, and Bax. In addition, they resulted in down-regulation of Bcl2 and a decrease in expression levels of cyclin D1, cyclin E, and ERBB2 genes. Cancer cells were arrested in the G0/G1 phase of the cell cycle. The results of immunocytochemistry showed significant down-regulation of cyclin D1 protein during the 48 hours treatment with bacterial supernatant compared to the untreated cells. The supernatant of probiotic has a great potential to inhibit the proliferation of HT-29 cells and the induction of apoptosis. might be used as a biological anti-cancer factor in the prevention and treatment of colon cancer.

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