Effect of Gamma Irradiation on Filtering Facepiece Respirators and SARS-CoV-2 Detection

Overview

Journal Sci Rep

Specialty Science

Date 2021 Oct 7

PMID 34615977

Citations 4

Authors

Khaled Al-Hadyan

Ghazi Alsbeih

Najla Al-Harbi

Sara Bin Judia

Maha Al-Ghamdi

Akram Almousa

Ibtihaj Alsharif

Razan Bakheet

Khaldoun Al-Romaih

Maha Al-Mozaini

Salem Al-Ghamdi

Belal Moftah

Rashed Alhmaid

Affiliations

Soon will be listed here.

Abstract

To cope with the shortage of filtering facepiece respirators (FFRs) during the coronavirus (COVID-19) pandemic, healthcare institutions were forced to reuse FFRs after applying different decontamination methods including gamma-irradiation (GIR). The aim of this study was to evaluate the effect of GIR on the filtration efficiency (FE) of FFRs and on SARS-CoV-2 detection. The FE of 2 FFRs types (KN95 and N95-3 M masks) was assessed at different particle sizes (0.3-5 µm) following GIR (0-15 kGy) delivered at either typical (1.65 kGy/h) or low (0.5088 kGy/h) dose rates. The detection of two SARS-CoV-2 RNA genes (E and RdRp4) following GIR (0-50 kGy) was carried out using RT-qPCR assay. Both masks showed an overall significant (P < 0.001) reduction in FE with increased GIR doses. No significant differences were observed between GIR dose rates on FE. The GIR exhibited significant increases (P ≤ 0.001) in the cycle threshold values (ΔCt) of both genes, with no detection following high doses. In conclusion, complete degradation of SARS-CoV-2 RNA can be achieved by high GIR (≥ 30 kGy), suggesting its potential use in FFRs decontamination. However, GIR exhibited adverse effects on FE in dose- and particle size-dependent manners, rendering its use to decontaminate FFRs debatable.

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References

Ohshima H, Iida Y, Matsuda A, Kuwabara M . Damage induced by hydroxyl radicals generated in the hydration layer of gamma-irradiated frozen aqueous solution of DNA. J Radiat Res. 1996; 37(3):199-207. DOI: 10.1269/jrr.37.199. View

Al-Hadyan K, Alsbeih G, Nobah A, Lindstrom J, Falatah S, Faran N . In-House Filtration Efficiency Assessment of Vapor Hydrogen Peroxide Decontaminated Filtering Facepiece Respirators (FFRs). Int J Environ Res Public Health. 2021; 18(13). PMC: 8297238. DOI: 10.3390/ijerph18137169. View

Rodriguez-Martinez C, Sossa-Briceno M, Cortes J . Decontamination and reuse of N95 filtering facemask respirators: A systematic review of the literature. Am J Infect Control. 2020; 48(12):1520-1532. PMC: 7342027. DOI: 10.1016/j.ajic.2020.07.004. View

Korystov YuN . Contributions of the direct and indirect effects of ionizing radiation to reproductive cell death. Radiat Res. 1992; 129(2):228-34. View

Beniac D, Andonov A, Grudeski E, Booth T . Architecture of the SARS coronavirus prefusion spike. Nat Struct Mol Biol. 2006; 13(8):751-2. PMC: 7097490. DOI: 10.1038/nsmb1123. View

Ward R . Mechanisms of poliovirus inactivation by the direct and indirect effects of ionizing radiation. Radiat Res. 1980; 83(2):330-44. View

Cai C, Floyd E . Effects of Sterilization With Hydrogen Peroxide and Chlorine Dioxide on the Filtration Efficiency of N95, KN95, and Surgical Face Masks. JAMA Netw Open. 2020; 3(6):e2012099. PMC: 7296389. DOI: 10.1001/jamanetworkopen.2020.12099. View

Derraik J, Anderson W, Connelly E, Anderson Y . Rapid Review of SARS-CoV-1 and SARS-CoV-2 Viability, Susceptibility to Treatment, and the Disinfection and Reuse of PPE, Particularly Filtering Facepiece Respirators. Int J Environ Res Public Health. 2020; 17(17). PMC: 7504573. DOI: 10.3390/ijerph17176117. View

Harrell C, Djonov V, Fellabaum C, Volarevic V . Risks of Using Sterilization by Gamma Radiation: The Other Side of the Coin. Int J Med Sci. 2018; 15(3):274-279. PMC: 5820857. DOI: 10.7150/ijms.22644. View

10.

Al-Saud H, Al-Romaih K, Bakheet R, Mahmoud L, Al-Harbi N, Alshareef I . Automated SARS-COV-2 RNA extraction from patient nasopharyngeal samples using a modified DNA extraction kit for high throughput testing. Ann Saudi Med. 2020; 40(5):373-381. PMC: 7511039. DOI: 10.5144/0256-4947.2020.373. View

11.

Thomas F, Davies A, Dulac G, Willis N, Papp-Vid G, Girard A . Gamma ray inactivation of some animal viruses. Can J Comp Med. 1981; 45(4):397-9. PMC: 1320171. View

12.

Feldmann F, Shupert W, Haddock E, Twardoski B, Feldmann H . Gamma Irradiation as an Effective Method for Inactivation of Emerging Viral Pathogens. Am J Trop Med Hyg. 2019; 100(5):1275-1277. PMC: 6493948. DOI: 10.4269/ajtmh.18-0937. View

13.

Rebmann T, Carrico R, Wang J . Physiologic and other effects and compliance with long-term respirator use among medical intensive care unit nurses. Am J Infect Control. 2013; 41(12):1218-23. PMC: 7132714. DOI: 10.1016/j.ajic.2013.02.017. View

14.

ELLIOTT L, McCormick J, Johnson K . Inactivation of Lassa, Marburg, and Ebola viruses by gamma irradiation. J Clin Microbiol. 1982; 16(4):704-8. PMC: 272450. DOI: 10.1128/jcm.16.4.704-708.1982. View

15.

Kumar M, Mazur S, Ork B, Postnikova E, Hensley L, Jahrling P . Inactivation and safety testing of Middle East Respiratory Syndrome Coronavirus. J Virol Methods. 2015; 223:13-8. PMC: 4555185. DOI: 10.1016/j.jviromet.2015.07.002. View

16.

POWERS E . Physical determinants of radiation sensitivity in bacterial spores. Adv Space Res. 1983; 3(8):73-8. DOI: 10.1016/0273-1177(83)90176-x. View

17.

Khang G, Lee H, Park J . Radiation effects on polypropylene for sterilization. Biomed Mater Eng. 1996; 6(5):323-34. View

18.

Sullivan R, Fassolitis A, Larkin E, Read Jr R, Peeler J . Inactivation of thirty viruses by gamma radiation. Appl Microbiol. 1971; 22(1):61-5. PMC: 377377. DOI: 10.1128/am.22.1.61-65.1971. View

19.

Jain R, Sarkale P, Mali D, Shete A, Patil D, Majumdar T . Inactivation of SARS-CoV-2 by gamma irradiation. Indian J Med Res. 2021; 153(1 & 2):196-198. PMC: 8184089. DOI: 10.4103/ijmr.IJMR_2789_20. View

20.

Hume A, Ames J, Rennick L, Duprex W, Marzi A, Tonkiss J . Inactivation of RNA Viruses by Gamma Irradiation: A Study on Mitigating Factors. Viruses. 2016; 8(7). PMC: 4974539. DOI: 10.3390/v8070204. View