Gamma Irradiation As an Effective Method for Inactivation of Emerging Viral Pathogens

Overview

Journal Am J Trop Med Hyg

Specialty Tropical Medicine

Date 2019 Mar 13

PMID 30860018

Citations 79

Authors

Friederike Feldmann

W Lesley Shupert

Elaine Haddock

Barri Twardoski

Heinz Feldmann

Affiliations

Soon will be listed here.

Abstract

Gamma irradiation using a cobalt-60 source is a commonly used method for the inactivation of infectious specimens to be handled safely in subsequent laboratory procedures. Here, we determined irradiation doses to safely inactivate liquid proteinaceous specimens harboring different emerging/reemerging viral pathogens known to cause neglected tropical and other diseases of regional or global public health importance. By using a representative arenavirus, bunyavirus, coronavirus, filovirus, flavivirus, orthomyxovirus, and paramyxovirus, we found that these enveloped viruses differed in their susceptibility to irradiation treatment with adsorbed doses for inactivation of a target dose of 1 × 10 50% tissue culture infectious dose (TCID)/mL ranging from 1 to 5 MRads. This finding seemed generally inversely correlated with genome size. Our data may help to guide other facilities in testing and verifying safe inactivation procedures.

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References

Ohshima H, Iida Y, Matsuda A, Kuwabara M . Damage induced by hydroxyl radicals generated in the hydration layer of gamma-irradiated frozen aqueous solution of DNA. J Radiat Res. 1996; 37(3):199-207. DOI: 10.1269/jrr.37.199. View

Kenny M, Albright K, Emery J, Bittle J . Inactivation of rubella virus by gamma radiation. J Virol. 1969; 4(6):807-10. PMC: 375942. DOI: 10.1128/JVI.4.6.807-810.1969. View

Ebihara H, Theriault S, Neumann G, Alimonti J, Geisbert J, Hensley L . In vitro and in vivo characterization of recombinant Ebola viruses expressing enhanced green fluorescent protein. J Infect Dis. 2007; 196 Suppl 2:S313-22. DOI: 10.1086/520590. View

ELLIOTT L, McCormick J, Johnson K . Inactivation of Lassa, Marburg, and Ebola viruses by gamma irradiation. J Clin Microbiol. 1982; 16(4):704-8. PMC: 272450. DOI: 10.1128/jcm.16.4.704-708.1982. View

Sullivan R, Fassolitis A, Larkin E, Read Jr R, Peeler J . Inactivation of thirty viruses by gamma radiation. Appl Microbiol. 1971; 22(1):61-5. PMC: 377377. DOI: 10.1128/am.22.1.61-65.1971. View

Ebihara H, Takada A, Kobasa D, Jones S, Neumann G, Theriault S . Molecular determinants of Ebola virus virulence in mice. PLoS Pathog. 2006; 2(7):e73. PMC: 1513261. DOI: 10.1371/journal.ppat.0020073. View

Haddock E, Feldmann F . Validating the Inactivation Effectiveness of Chemicals on Ebola Virus. Methods Mol Biol. 2017; 1628:251-257. DOI: 10.1007/978-1-4939-7116-9_20. View

Bray M, Davis K, Geisbert T, Schmaljohn C, Huggins J . A mouse model for evaluation of prophylaxis and therapy of Ebola hemorrhagic fever. J Infect Dis. 1998; 178(3):651-61. DOI: 10.1086/515386. View

Jordan R, Kempe L . Inactivation of some animal viruses with gamma radiation from cobalt-60. Proc Soc Exp Biol Med. 1956; 91(2):212-5. DOI: 10.3181/00379727-91-22215. View

10.

Lomax M, Folkes L, ONeill P . Biological consequences of radiation-induced DNA damage: relevance to radiotherapy. Clin Oncol (R Coll Radiol). 2013; 25(10):578-85. DOI: 10.1016/j.clon.2013.06.007. View

11.

House C, Mikiciuk P, Berninger M . Laboratory diagnosis of African horse sickness: comparison of serological techniques and evaluation of storage methods of samples for virus isolation. J Vet Diagn Invest. 1990; 2(1):44-50. DOI: 10.1177/104063879000200108. View

12.

Mitchell S, McCormick J . Physicochemical inactivation of Lassa, Ebola, and Marburg viruses and effect on clinical laboratory analyses. J Clin Microbiol. 1984; 20(3):486-9. PMC: 271356. DOI: 10.1128/jcm.20.3.486-489.1984. View

13.

Hume A, Ames J, Rennick L, Duprex W, Marzi A, Tonkiss J . Inactivation of RNA Viruses by Gamma Irradiation: A Study on Mitigating Factors. Viruses. 2016; 8(7). PMC: 4974539. DOI: 10.3390/v8070204. View

14.

Niedrig M, Donoso-Mantke O, Schadler R . The European Network for Diagnostics of Imported Viral Diseases (ENIVD)--12 years of strengthening the laboratory diagnostic capacity in Europe. Euro Surveill. 2007; 12(4):E070419.5. DOI: 10.2807/esw.12.16.03180-en. View

15.

Haddock E, Feldmann F, Feldmann H . Effective Chemical Inactivation of Ebola Virus. Emerg Infect Dis. 2016; 22(7):1292-4. PMC: 4918181. DOI: 10.3201/eid2207.160233. View