Breath Biomarkers in Diagnostic Applications

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2021 Sep 28

PMID 34576985

Citations 19

Authors

Y Lan Pham

Jonathan Beauchamp

Affiliations

Soon will be listed here.

Abstract

The detection of chemical compounds in exhaled human breath presents an opportunity to determine physiological state, diagnose disease or assess environmental exposure. Recent advancements in metabolomics research have led to improved capabilities to explore human metabolic profiles in breath. Despite some notable challenges in sampling and analysis, exhaled breath represents a desirable medium for metabolomics applications, foremost due to its non-invasive, convenient and practically limitless availability. Several breath-based tests that target either endogenous or exogenous gas-phase compounds are currently established and are in practical and/or clinical use. This review outlines the concept of breath analysis in the context of these unique tests and their applications. The respective breath biomarkers targeted in each test are discussed in relation to their physiological production in the human body and the development and implementation of the associated tests. The paper concludes with a brief insight into prospective tests and an outlook of the future direction of breath research.

Citing Articles

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References

Heffler E, Carpagnano G, Favero E, Guida G, Maniscalco M, Motta A . Fractional Exhaled Nitric Oxide (FENO) in the management of asthma: a position paper of the Italian Respiratory Society (SIP/IRS) and Italian Society of Allergy, Asthma and Clinical Immunology (SIAAIC). Multidiscip Respir Med. 2020; 15(1):36. PMC: 7137762. DOI: 10.4081/mrm.2020.36. View

de Lacy Costello B, Amann A, Al-Kateb H, Flynn C, Filipiak W, Khalid T . A review of the volatiles from the healthy human body. J Breath Res. 2014; 8(1):014001. DOI: 10.1088/1752-7155/8/1/014001. View

Sakka S . Assessing liver function. Curr Opin Crit Care. 2007; 13(2):207-14. DOI: 10.1097/MCC.0b013e328012b268. View

Kalant H . Research on tolerance: what can we learn from history?. Alcohol Clin Exp Res. 1998; 22(1):67-76. DOI: 10.1111/j.1530-0277.1998.tb03618.x. View

Beauchamp J, Pleil J . Simply breath-taking? Developing a strategy for consistent breath sampling. J Breath Res. 2013; 7(4):042001. DOI: 10.1088/1752-7155/7/4/042001. View

Vaira D, Vakil N . Blood, urine, stool, breath, money, and Helicobacter pylori. Gut. 2001; 48(3):287-9. PMC: 1760150. DOI: 10.1136/gut.48.3.287. View

Braden B . Methods and functions: Breath tests. Best Pract Res Clin Gastroenterol. 2009; 23(3):337-52. DOI: 10.1016/j.bpg.2009.02.014. View

Silkoff P, Carlson M, Bourke T, Katial R, Ogren E, Szefler S . The Aerocrine exhaled nitric oxide monitoring system NIOX is cleared by the US Food and Drug Administration for monitoring therapy in asthma. J Allergy Clin Immunol. 2004; 114(5):1241-56. DOI: 10.1016/j.jaci.2004.08.042. View

Graham D, Malaty H, Cole R, Martin R, KLEIN P . Simplified 13C-urea breath test for detection of Helicobacter pylori infection. Am J Gastroenterol. 2001; 96(6):1741-5. DOI: 10.1111/j.1572-0241.2001.03867.x. View

10.

Lee J, Camilleri M, Zinsmeister A, Burton D, Kost L, KLEIN P . A valid, accurate, office based non-radioactive test for gastric emptying of solids. Gut. 2000; 46(6):768-73. PMC: 1756454. DOI: 10.1136/gut.46.6.768. View

11.

Camilleri M, Chedid V, Ford A, Haruma K, Horowitz M, Jones K . Gastroparesis. Nat Rev Dis Primers. 2018; 4(1):41. DOI: 10.1038/s41572-018-0038-z. View

12.

Phillips M, Boehmer J, Cataneo R, Cheema T, Eisen H, Fallon J . Prediction of heart transplant rejection with a breath test for markers of oxidative stress. Am J Cardiol. 2004; 94(12):1593-4. DOI: 10.1016/j.amjcard.2004.08.052. View

13.

Horvath I, Barnes P, Loukides S, Sterk P, Hogman M, Olin A . A European Respiratory Society technical standard: exhaled biomarkers in lung disease. Eur Respir J. 2017; 49(4). DOI: 10.1183/13993003.00965-2016. View

14.

Tornberg D, Bjorne H, Lundberg J, Weitzberg E . Multiple single-breath measurements of nitric oxide in the intubated patient. Am J Respir Crit Care Med. 2003; 168(10):1210-5. DOI: 10.1164/rccm.200306-784OC. View

15.

Kharitonov S, Yates D, Robbins R, Shinebourne E, Barnes P . Increased nitric oxide in exhaled air of asthmatic patients. Lancet. 1994; 343(8890):133-5. DOI: 10.1016/s0140-6736(94)90931-8. View

16.

Kerlin P, Wong L, Harris B, Capra S . Rice flour, breath hydrogen, and malabsorption. Gastroenterology. 1984; 87(3):578-85. View

17.

Price D, Buhl R, Chan A, Freeman D, Gardener E, Godley C . Fractional exhaled nitric oxide as a predictor of response to inhaled corticosteroids in patients with non-specific respiratory symptoms and insignificant bronchodilator reversibility: a randomised controlled trial. Lancet Respir Med. 2017; 6(1):29-39. DOI: 10.1016/S2213-2600(17)30424-1. View

18.

Basanta M, Ibrahim B, Dockry R, Douce D, Morris M, Singh D . Exhaled volatile organic compounds for phenotyping chronic obstructive pulmonary disease: a cross-sectional study. Respir Res. 2012; 13:72. PMC: 3514190. DOI: 10.1186/1465-9921-13-72. View

19.

Heading R . Gastric emptying: a clinical perspective. Clin Sci (Lond). 1982; 63(3):231-5. DOI: 10.1042/cs0630231. View

20.

Hogman M, Stromberg S, Schedin U, Frostell C, Hedenstierna G, Gustafsson L . Nitric oxide from the human respiratory tract efficiently quantified by standardized single breath measurements. Acta Physiol Scand. 1997; 159(4):345-6. DOI: 10.1046/j.1365-201X.1997.00101.x. View