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Generation and Analysis of Mosaic Spinal Cord Organoids Derived from Mouse Embryonic Stem Cells

Overview
Journal Methods Mol Biol
Specialty Molecular Biology
Date 2021 Sep 25
PMID 34562238
Citations 2
Authors
Carina Jagers
Henk Roelink
Affiliations
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Abstract

Three central cell populations play roles in morphogen action, the cells that produce, the cells that distribute, and the cells that respond to the morphogen. Taking advantage of the properties of embryonic stem cell to aggregate and readily differentiate into neural progenitor tissue, we describe an approach using genetically modified murine stem cell lines to individually address the contribution of these cells in the establishment and response to a morphogenetic gradient in mosaic spinal cord organoids.

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