Generating Cerebral Organoids from Human Pluripotent Stem Cells

Overview

Journal Methods Mol Biol

Specialty Molecular Biology

Date 2021 Sep 24

PMID 34558011

Citations 3

Authors

Leon Chew

Adam Anonuevo

Erin Knock

Affiliations

Soon will be listed here.

Abstract

3D brain organoids derived from human pluripotent stem cells (hPSCs) possess the remarkable ability to self-organize and differentiate into tissue resembling the early human fetal brain. Brain organoids provide a powerful tool for studying human brain development and disease in an in vitro system. Here we describe a protocol for the differentiation of hPSCs to human cerebral organoids using a commercially available kit (STEMdiff™ Cerebral Organoid Kit) and discuss methods to scale up the protocol in a high-throughput manner.

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