Introduction of Genes into Preimplantation Mouse Embryos by Use of a Defective Recombinant Retrovirus

Overview

Journal Proc Natl Acad Sci U S A

Specialty Science

Date 1986 Jan 1

PMID 3455774

Citations 10

Authors

J L Rubenstein

J F Nicolas

F Jacob

Affiliations

Soon will be listed here.

Abstract

Two-cell and four-cell preimplantation mouse embryos were cocultured in vitro with fibroblasts producing the recombinant retrovirus M-MuLV-neo. No wildtype helper virus was detected in these cultures. Of the embryos that survived the in vitro cultivation and the reimplantation into foster mothers, 2 of 15 that were tested contained the proviral genome. The provirus integrated as a single copy at a unique site. We estimate that approximately equal to 20% of the cells in each of the two transgenic fetuses contained the provirus.

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