Alpha 2.0
Login Register
» Articles » PMID: 34541170

Exit from Pluripotency Assay of Mouse Embryonic Stem Cells

Overview
Journal Bio Protoc
Specialty Biology
Date 2021 Sep 20
PMID 34541170
Citations 2
Authors
Daniel Cirera-Salinas
Constance Ciaudo
Affiliations
Soon will be listed here.
Abstract

A novel method to assess the dissolution of the core pluripotency transcription-factor circuit of mouse Embryonic Stem Cells (mESCs) has been developed ( Ying , 2003 ; Betschinger , 2013 ). In order to efficiently identify genes essential for the break-down of the pluripotency network in mutant mESCs with proliferation defects, we adapted this 'exit from pluripotency assay' ( Bodak , 2017 ; Cirera-Salinas , 2017 ). The protocol described here has been successfully applied to several mESC lines and is easily transposable from one laboratory to another.

Citing Articles

Understanding the interplay of membrane trafficking, cell surface mechanics, and stem cell differentiation.

Li J, Trivedi V, Diz-Munoz A Semin Cell Dev Biol. 2022; 133:123-134.

PMID: 35641408 PMC: 9703995. DOI: 10.1016/j.semcdb.2022.05.010.

Cell Surface Mechanics Gate Embryonic Stem Cell Differentiation.

Bergert M, Lembo S, Sharma S, Russo L, Milovanovic D, Gretarsson K Cell Stem Cell. 2020; 28(2):209-216.e4.

PMID: 33207217 PMC: 7875094. DOI: 10.1016/j.stem.2020.10.017.

References
1.
Bodak M, Cirera-Salinas D, Yu J, Ngondo R, Ciaudo C . , a new regulator of pluripotency exit and LINE-1 elements in mouse embryonic stem cells. FEBS Open Bio. 2017; 7(2):204-220. PMC: 5292673. DOI: 10.1002/2211-5463.12174. View
2.
Ying Q, Wray J, Nichols J, Batlle-Morera L, Doble B, Woodgett J . The ground state of embryonic stem cell self-renewal. Nature. 2008; 453(7194):519-23. PMC: 5328678. DOI: 10.1038/nature06968. View
3.
Betschinger J, Nichols J, Dietmann S, Corrin P, Paddison P, Smith A . Exit from pluripotency is gated by intracellular redistribution of the bHLH transcription factor Tfe3. Cell. 2013; 153(2):335-47. PMC: 3661979. DOI: 10.1016/j.cell.2013.03.012. View
4.
Cirera-Salinas D, Yu J, Bodak M, Ngondo R, Herbert K, Ciaudo C . Noncanonical function of DGCR8 controls mESC exit from pluripotency. J Cell Biol. 2017; 216(2):355-366. PMC: 5294780. DOI: 10.1083/jcb.201606073. View
5.
Ying Q, Stavridis M, Griffiths D, Li M, Smith A . Conversion of embryonic stem cells into neuroectodermal precursors in adherent monoculture. Nat Biotechnol. 2003; 21(2):183-6. DOI: 10.1038/nbt780. View