, a New Regulator of Pluripotency Exit and LINE-1 Elements in Mouse Embryonic Stem Cells

Overview

Journal FEBS Open Bio

Specialty Biology

Date 2017 Feb 9

PMID 28174687

Citations 25

Authors

Maxime Bodak

Daniel Cirera-Salinas

Jian Yu

Richard P Ngondo

Constance Ciaudo

Affiliations

Soon will be listed here.

Abstract

A gene regulation network orchestrates processes ensuring the maintenance of cellular identity and genome integrity. Small RNAs generated by the RNAse III DICER have emerged as central players in this network. Moreover, deletion of in mice leads to early embryonic lethality. To better understand the underlying mechanisms leading to this phenotype, we generated -deficient mouse embryonic stem cells (mESCs). Their detailed characterization revealed an impaired differentiation potential, and incapacity to exit from the pluripotency state. We also observed a strong accumulation of LINE-1 (L1s) transcripts, which was translated at protein level and led to an increased L1s retrotransposition. Our findings reveal as a new essential player that sustains mESCs self-renewal and genome integrity by controlling L1s regulation.

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