Locating Chemical Modifications in RNA Sequences Through Ribonucleases and LC-MS Based Analysis

Overview

Journal Methods Enzymol

Specialty Biochemistry

Date 2021 Sep 14

PMID 34517943

Citations 5

Authors

Priti Thakur

Scott Abernathy

Patrick A Limbach

Balasubrahmanyam Addepalli

Affiliations

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Abstract

Knowledge of the structural information is essential for understanding the functional details of modified RNA. Cellular non-coding RNA such as rRNA, tRNA and even viral RNAs contain a number of post-transcriptional modifications with varied degree of diversity and density. In this chapter, we discuss the use of a combination of biochemical and analytical tools such as ribonucleases and liquid chromatography coupled with mass spectrometry approaches for characterization of modified RNA. We present the protocols and alternate strategies for obtaining confident modified sequence information to facilitate the understanding of function.

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