Live-cell Imaging Reveals Square Shape Spindles and Long Mitosis Duration in the Silkworm Holocentric Cells

Overview

Journal MicroPubl Biol

Specialty Biology

Date 2021 Sep 13

PMID 34514356

Citations 4

Authors

Lucien Vanpoperinghe

Frederique Carlier-Grynkorn

Gaetan Cornilleau

Takahiro Kusakabe

Ines A Drinnenberg

Phong T Tran

Affiliations

Soon will be listed here.

Abstract

Proper chromosome segregation during mitosis requires both the assembly of a microtubule (MT)-based spindle and the assembly of DNA-centromere-based kinetochore structure. Kinetochore-to-MT attachment enables chromosome separation. Monocentric cells, such as found in human, have one unique kinetochore per chromosome. Holocentric cells, such as found in the silkworm, in contrast, have multiple kinetochore structures per chromosome. Interestingly, some human cancer chromosomes contain more than one kinetochore, a condition called di- and tricentric. Thus, comparing how wild-type mono- and holocentric cells perform mitosis may provide novel insights into cancer di- and tricentric cell mitosis. We present here live-cell imaging of human RPE1 and silkworm BmN4 cells, revealing striking differences in spindle architecture and dynamics, and highlighting differential kinesin function between mono- and holocentric cells.

Citing Articles

Ipsilateral restriction of chromosome movement along a centrosome, and apical-basal axis during the cell cycle.

Cai P, Casas C, Quintero Plancarte G, Mikawa T, Hua L Chromosome Res. 2025; 33(1):1.

PMID: 39751905 PMC: 11698895. DOI: 10.1007/s10577-024-09760-0.

Ipsilateral restriction of chromosome movement along a centrosome, and apical-basal axis during the cell cycle.

Cai P, Casas C, Quintero Plancarte G, Mikawa T, Hua L Res Sq. 2024; .

PMID: 38746098 PMC: 11092853. DOI: 10.21203/rs.3.rs-4283973/v1.

siRNA-Mediated BmAurora B Depletion Impedes the Formation of Holocentric Square Spindles in Silkworm Metaphase BmN4 Cells.

Zhang B, Ayra-Pardo C, Liu X, Song M, Li D, Kan Y Insects. 2024; 15(1).

PMID: 38276821 PMC: 10817069. DOI: 10.3390/insects15010072.

Kinesin-5 and kinesin-14 are partially antagonistic in spindle assembly in the holocentric silkworm cells.

Kassamaly I, Cornilleau G, Drinnenberg I, Tran P MicroPubl Biol. 2022; 2022.

PMID: 36082020 PMC: 9445969. DOI: 10.17912/micropub.biology.000630.

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