Identification of Distinct Tumor Cell Populations and Key Genetic Mechanisms Through Single Cell Sequencing in Hepatoblastoma

Overview

Journal Commun Biol

Specialty Biology

Date 2021 Sep 9

PMID 34497364

Citations 16

Authors

Alexander Bondoc

Kathryn Glaser

Kang Jin

Charissa Lake

Stefano Cairo

James Geller

Gregory Tiao

Bruce Aronow

Affiliations

Soon will be listed here.

Abstract

Hepatoblastoma (HB) is the most common primary liver malignancy of childhood, and molecular investigations are limited and effective treatment options for chemoresistant disease are lacking. There is a knowledge gap in the investigation of key driver cells of HB in tumor. Here we show single cell ribonucleic acid sequencing (scRNAseq) analysis of human tumor, background liver, and patient derived xenograft (PDX) to demonstrate gene expression patterns within tumor and to identify intratumor cell subtype heterogeneity to define differing roles in pathogenesis based on intracellular signaling in pediatric HB. We have identified a driver tumor cell cluster in HB by genetic expression which can be examined to define disease mechanism and treatments. Identification of both critical mechanistic pathways combined with unique cell populations provide the basis for discovery and investigation of novel treatment strategies in vitro and in vivo.

Citing Articles

Single-Cell RNA Sequencing Reveals LEF1-Driven Wnt Pathway Activation as a Shared Oncogenic Program in Hepatoblastoma and Medulloblastoma.

Desterke C, Fu Y, Bonifacio-Mundaca J, Monge C, Pineau P, Mata-Garrido J Curr Oncol. 2025; 32(1).

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Specifying cellular context of transcription factor regulons for exploring context-specific gene regulation programs.

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Characterization of an Activated Metabolic Transcriptional Program in Hepatoblastoma Tumor Cells Using scRNA-seq.

Monge C, Frances R, Marchio A, Pineau P, Desterke C, Mata-Garrido J Int J Mol Sci. 2024; 25(23).

PMID: 39684755 PMC: 11641740. DOI: 10.3390/ijms252313044.

A developmental biliary lineage program cooperates with Wnt activation to promote cell proliferation in hepatoblastoma.

Wu P, Fish M, Hazard F, Zhu C, Vennam S, Walton H Nat Commun. 2024; 15(1):10007.

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First Transcriptome Analysis of Hepatoblastoma in Brazil: Unraveling the Pivotal Role of Noncoding RNAs and Metabolic Pathways.

Aguiar T, Rivas M, Silva E, Pires S, Dangoni G, Macedo T Biochem Genet. 2024; .

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