Chromosomal Characterization of Revealed by Oligo-FISH

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2021 Aug 27

PMID 34445245

Citations 6

Authors

Fan Yu

Jin Chai

Xueting Li

Zehuai Yu

Ruiting Yang

Xueer Ding

Qiusong Wang

Jiayun Wu

Xiping Yang

Zuhu Deng

Affiliations

Soon will be listed here.

Abstract

Sugarcane is of important economic value for producing sugar and bioethanol.   (old name:  ) is an intergeneric wild species of sugarcane that has desirable resistance traits for improving sugarcane varieties. However, the scarcity of chromosome markers has hindered the cytogenetic study of . Here we applied maize chromosome painting probes (MCPs) to identify chromosomes in sorghum and using a repeated fluorescence in situ hybridization (FISH) system. Sequential FISH revealed that these MCPs can be used as reliable chromosome markers for , even though has diverged from maize over 18 MYs (million years). Using these MCPs, we identified chromosomes based on their sequence similarity compared to sorghum and labeled them 1 through 10. Then, the karyotype of was established by multiple oligo-FISH. Furthermore, FISH results revealed that 5S rDNA and 35S rDNA are localized on chromosomes 5 and 6, respectively, in . Altogether, these results represent an essential step for further cytogenetic research of in sugarcane breeding.

Citing Articles

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