Development of a Duck Genomic Reference Material by Digital PCR Platforms for the Detection of Meat Adulteration

Overview

Journal Foods

Specialty Biotechnology

Date 2021 Aug 27

PMID 34441667

Citations 3

Authors

Xiaoyun Chen

Yi Ji

Kai Li

Xiaofu Wang

Cheng Peng

Xiaoli Xu

Xinwu Pei

Junfeng Xu

Liang Li

Affiliations

Soon will be listed here.

Abstract

Low-cost meat, such as duck, is frequently used to adulterate more expensive foods like lamb or beef in many countries. However, the lack of DNA-based reference materials has limited the quality control and detection of adulterants. Here, we report the development and validation of duck genomic DNA certified reference materials (CRMs) through the detection of the duck () gene by digital PCR (dPCR) for the identification of duck meat in food products. The certified value of in CRMs was 5.78 ± 0.51 × 10 copies/μL with extended uncertainty (coverage factor = 2) based on quantification by eight independent collaborating laboratories. Quantification of the mitochondrial gene revealed a concentration of 2.0 × 10 copies/μL, as an information value. The CRMs were also used to determine the limit of detection (LOD) for six commercial testing kits, which confirmed that these kits meet or exceed their claimed sensitivity and are reliable for duck detection.

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References

Kim M, Lee Y, Suh S, Kim H . Species Identification of Red Deer (), Roe Deer (), and Water Deer () Using Capillary Electrophoresis-Based Multiplex PCR. Foods. 2020; 9(8). PMC: 7466337. DOI: 10.3390/foods9080982. View

Dong L, Meng Y, Wang J, Liu Y . Evaluation of droplet digital PCR for characterizing plasmid reference material used for quantifying ammonia oxidizers and denitrifiers. Anal Bioanal Chem. 2014; 406(6):1701-12. PMC: 3936116. DOI: 10.1007/s00216-013-7546-1. View

Floren C, Wiedemann I, Brenig B, Schutz E, Beck J . Species identification and quantification in meat and meat products using droplet digital PCR (ddPCR). Food Chem. 2014; 173:1054-8. DOI: 10.1016/j.foodchem.2014.10.138. View

Bohme K, Calo-Mata P, Barros-Velazquez J, Ortea I . Review of Recent DNA-Based Methods for Main Food-Authentication Topics. J Agric Food Chem. 2019; 67(14):3854-3864. DOI: 10.1021/acs.jafc.8b07016. View

Yang Y, Li L, Yang H, Li X, Zhang X, Xu J . Development of Certified Matrix-Based Reference Material as a Calibrator for Genetically Modified Rice G6H1 Analysis. J Agric Food Chem. 2018; 66(14):3708-3715. DOI: 10.1021/acs.jafc.8b00468. View

Burns M, Wiseman G, Knight A, Bramley P, Foster L, Rollinson S . Measurement issues associated with quantitative molecular biology analysis of complex food matrices for the detection of food fraud. Analyst. 2015; 141(1):45-61. DOI: 10.1039/c5an01392e. View

Li J, Li L, Zhang L, Zhang X, Li X, Zhai S . Development of a certified genomic DNA reference material for detection and quantification of genetically modified rice KMD. Anal Bioanal Chem. 2020; 412(25):7007-7016. DOI: 10.1007/s00216-020-02834-y. View

He H, Das B, Cleveland M, Chen L, Camalier C, Liu L . Development and interlaboratory evaluation of a NIST Reference Material RM 8366 for EGFR and MET gene copy number measurements. Clin Chem Lab Med. 2019; 57(8):1142-1152. PMC: 6875440. DOI: 10.1515/cclm-2018-1306. View

Hossain M, Ali M, Hamid S, Asing , Mustafa S, Desa M . Double Gene Targeting Multiplex Polymerase Chain Reaction-Restriction Fragment Length Polymorphism Assay Discriminates Beef, Buffalo, and Pork Substitution in Frankfurter Products. J Agric Food Chem. 2016; 64(32):6343-54. DOI: 10.1021/acs.jafc.6b02224. View

10.

Vallejo C, Tere C, Calderon M, Arias M, Leguizamon J . Development of a genomic DNA reference material for Salmonella enteritidis detection using polymerase chain reaction. Mol Cell Probes. 2020; 55:101690. DOI: 10.1016/j.mcp.2020.101690. View

11.

Cao Y, Zheng K, Jiang J, Wu J, Shi F, Song X . A novel method to detect meat adulteration by recombinase polymerase amplification and SYBR green I. Food Chem. 2018; 266:73-78. DOI: 10.1016/j.foodchem.2018.05.115. View

12.

Li J, Zhang L, Li L, Li X, Zhang X, Zhai S . Development of Genomic DNA Certified Reference Materials for Genetically Modified Rice Kefeng 6. ACS Omega. 2020; 5(34):21602-21609. PMC: 7469412. DOI: 10.1021/acsomega.0c02274. View

13.

Li T, Wang J, Wang Z, Qiao L, Liu R, Li S . Quantitative determination of mutton adulteration with single-copy nuclear genes by real-time PCR. Food Chem. 2020; 344:128622. DOI: 10.1016/j.foodchem.2020.128622. View

14.

Seddaoui N, Amine A . Smartphone-based competitive immunoassay for quantitative on-site detection of meat adulteration. Talanta. 2021; 230:122346. DOI: 10.1016/j.talanta.2021.122346. View

15.

Xu J, Qu S, Sun N, Zhang W, Zhang J, Song Q . Construction of a reference material panel for detecting //// mutations in plasma ctDNA. J Clin Pathol. 2020; 74(5):314-320. PMC: 8070650. DOI: 10.1136/jclinpath-2020-206745. View

16.

Dong L, Wang X, Wang S, Du M, Niu C, Yang J . Interlaboratory assessment of droplet digital PCR for quantification of BRAF V600E mutation using a novel DNA reference material. Talanta. 2019; 207:120293. DOI: 10.1016/j.talanta.2019.120293. View

17.

Lopez-Oceja A, Nunez C, Baeta M, Gamarra D, de Pancorbo M . Species identification in meat products: A new screening method based on high resolution melting analysis of cyt b gene. Food Chem. 2017; 237:701-706. DOI: 10.1016/j.foodchem.2017.06.004. View

18.

Xu R, Wei S, Zhou G, Ren J, Liu Z, Tang S . Multiplex TaqMan locked nucleic acid real-time PCR for the differential identification of various meat and meat products. Meat Sci. 2017; 137:41-46. DOI: 10.1016/j.meatsci.2017.11.003. View

19.

Lin L, Zheng Y, Huang H, Zhuang F, Chen H, Zha G . A visual method to detect meat adulteration by recombinase polymerase amplification combined with lateral flow dipstick. Food Chem. 2021; 354:129526. DOI: 10.1016/j.foodchem.2021.129526. View

20.

Kumar A, Kumar R, Sharma B, Gokulakrishnan P, Mendiratta S, Sharma D . Identification of species origin of meat and meat products on the DNA basis: a review. Crit Rev Food Sci Nutr. 2014; 55(10):1340-51. DOI: 10.1080/10408398.2012.693978. View