DNA Synthesis in Purified Maize Mitochondria

Overview

Journal Curr Genet

Specialty Genetics

Date 1986 Jan 1

PMID 3442831

Citations 6

Authors

P Bedinger

V Walbot

Affiliations

Soon will be listed here.

Abstract

We have developed an in organelle DNA synthesis system using isolated mitochondria from maize. The organelles used in this assay are shown to be intact by a number of criteria. Both the high molecular weight components and the smaller plasmid-like components of the mitochondrial genome are used as templates; however, the plasmid-like elements are relatively more active as templates. The termini of the linear plasmids--S-1, S-2 and the 2.3 kbp plasmids--are more highly labelled than internal regions, probably as a result of filling in of gaps at the termini mediated by a DNA polymerase or to exonuclease degradation of the 3' OH termini, with subsequent filling in. Although most of the DNA synthesis observed in this system most likely results from this type of synthesis at DNA nicks or termini, a small amount of specific, potentially replication-associated, synthesis is also detected.

Citing Articles

Purification and characterization of a gamma-like DNA polymerase from Chenopodium album L.

Meissner K, Heinhorst S, Cannon G, Borner T Nucleic Acids Res. 1993; 21(21):4893-9.

PMID: 8177736 PMC: 311402. DOI: 10.1093/nar/21.21.4893.

In organello replication and viral affinity of linear, extrachromosomal DNA of the ascomycete Ascobolus immersus.

Kempken F, Meinhardt F, Esser K Mol Gen Genet. 1989; 218(3):523-30.

PMID: 2573821 DOI: 10.1007/BF00332419.

Isolation from wheat mitochondria of a membrane-associated high molecular weight complex involved in DNA synthesis.

Echeverria M, Robert D, Carde J, Litvak S Plant Mol Biol. 1991; 16(2):301-15.

PMID: 1893101 DOI: 10.1007/BF00020561.

High-frequency transfer of linear DNA containing 5'-covalently linked terminal proteins: electroporation of bacteriophage PRD1 genome into Escherichia coli.

Lyra C, Savilahti H, Bamford D Mol Gen Genet. 1991; 228(1-2):65-9.

PMID: 1886619 DOI: 10.1007/BF00282449.

Transcriptional and posttranscriptional regulation of maize mitochondrial gene expression.

Mulligan R, Leon P, Walbot V Mol Cell Biol. 1991; 11(1):533-43.

PMID: 1846025 PMC: 359662. DOI: 10.1128/mcb.11.1.533-543.1991.

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