S1 and S2, the Linear Mitochondrial DNAs Present in a Male Sterile Line of Maize, Possess Terminally Attached Proteins

Overview

Journal Nucleic Acids Res

Publisher Oxford University Press

Specialty Biochemistry

Date 1982 Dec 20

PMID 7162989

Citations 47

Authors

R J Kemble

R D Thompson

Affiliations

Soon will be listed here.

Abstract

S1 and S2 are short linear mitochondrial DNA molecules found in a particular male sterile cytoplasm of maize. We show here that these DNA molecules and two other related linear DNA species found in maize mitochondria, have proteins attached, probably covalently, to their 5' ends. This is the first demonstration of such a linear DNA-terminal protein association in higher eukaryotes. Such proteins may be involved in priming replication of these DNAs.

Citing Articles

Variable presence of the 1.94kb mitochondrial plasmid in maize S cytoplasm and its relationship to cytoplasmic male sterility.

Carlson J, Kemble R Plant Mol Biol. 2013; 4(2-3):117-23.

PMID: 24310748 DOI: 10.1007/BF02418758.

Properties of the linear N1 and N2 plasmid-like DNAs from mitochondria of cytoplasmic male-sterile Sorghum bicolor.

Chase C, Pring D Plant Mol Biol. 2013; 6(1):53-64.

PMID: 24307154 DOI: 10.1007/BF00021306.

Transcription of the S-2 maize mitochondrial plasmid.

Traynor P, Levings 3rd C Plant Mol Biol. 2013; 7(4):255-63.

PMID: 24302368 DOI: 10.1007/BF00752899.

A protein fraction stably linked to DNA in plant chromatin.

Avramova Z, Ivanchenko M, TSANEV R Plant Mol Biol. 2013; 11(4):401-8.

PMID: 24272396 DOI: 10.1007/BF00039020.

Cytoplasmic DNA variation in a potato protoclonal population.

Kemble R, Shepard J Theor Appl Genet. 2013; 69(2):211-6.

PMID: 24253714 DOI: 10.1007/BF00272897.

References

Lonsdale D, Thompson R, Hodge T . The integrated forms of the S1 and S2 DNA elements of maize male sterile mitochondrial DNA are flanked by a large repeated sequence. Nucleic Acids Res. 1981; 9(15):3657-69. PMC: 327382. DOI: 10.1093/nar/9.15.3657. View

Challberg M, Desiderio S, Kelly Jr T . Adenovirus DNA replication in vitro: characterization of a protein covalently linked to nascent DNA strands. Proc Natl Acad Sci U S A. 1980; 77(9):5105-9. PMC: 350005. DOI: 10.1073/pnas.77.9.5105. View

Pring D, Levings C, Hu W, Timothy D . Unique DNA associated with mitochondria in the "S"-type cytoplasm of male-sterile maize. Proc Natl Acad Sci U S A. 1977; 74(7):2904-8. PMC: 431340. DOI: 10.1073/pnas.74.7.2904. View

Tu C, Cohen S . 3'-end labeling of DNA with [alpha-32P]cordycepin-5'-triphosphate. Gene. 1980; 10(2):177-83. DOI: 10.1016/0378-1119(80)90135-3. View

Salas M, Mellado R, Vinuela E . Characterization of a protein covalently linked to the 5' termini of the DNA of Bacillus subtilis phage phi29. J Mol Biol. 1978; 119(2):269-91. DOI: 10.1016/0022-2836(78)90438-2. View

Kim B, Mans R, Conde M, Pring D, Levings 3rd C . Physical mapping of homologous segments of mitochondrial episomes from S male-sterile maize. Plasmid. 1982; 7(1):1-14. DOI: 10.1016/0147-619x(82)90022-1. View

Rekosh D, Russell W, Bellet A, Robinson A . Identification of a protein linked to the ends of adenovirus DNA. Cell. 1977; 11(2):283-95. DOI: 10.1016/0092-8674(77)90045-9. View

Levings 3rd C, Kim B, Pring D, Conde M, Mans R, Laughnan J . Cytoplasmic Reversion of cms-S in Maize: Association with a Transpositional Event. Science. 1980; 209(4460):1021-3. DOI: 10.1126/science.209.4460.1021. View

Kemble R, Gunn R, Flavell R . Classification of Normal and Male-Sterile Cytoplasms in Maize. II. Electrophoretic Analysis of DNA Species in Mitochondria. Genetics. 1980; 95(2):451-8. PMC: 1214238. DOI: 10.1093/genetics/95.2.451. View

10.

Forde B, Oliver R, Leaver C, Gunn R, Kemble R . Classification of normal and male-sterile cytoplasms in maize. I. Electrophoretic analysis of variation in mitochondrially synthesized proteins. Genetics. 1980; 95(2):443-50. PMC: 1214237. DOI: 10.1093/genetics/95.2.443. View

11.

Thompson R, Kemble R, Flavell R . Variations in mitochondrial DNA organisation between normal and male-sterile cytoplasms of maize. Nucleic Acids Res. 1980; 8(9):1999-2008. PMC: 324053. DOI: 10.1093/nar/8.9.1999. View

12.

Maxam A, Gilbert W . Sequencing end-labeled DNA with base-specific chemical cleavages. Methods Enzymol. 1980; 65(1):499-560. DOI: 10.1016/s0076-6879(80)65059-9. View

13.

Hirochika H, Sakaguchi K . Analysis of linear plasmids isolated from Streptomyces: association of protein with the ends of the plasmid DNA. Plasmid. 1982; 7(1):59-65. DOI: 10.1016/0147-619x(82)90027-0. View