Improved Functional Properties of Meat Analogs by Laccase Catalyzed Protein and Pectin Crosslinks

Overview

Journal Sci Rep

Specialty Science

Date 2021 Aug 18

PMID 34404846

Citations 17

Authors

Kiyota Sakai

Yukihide Sato

Masamichi Okada

Shotaro Yamaguchi

Affiliations

Soon will be listed here.

Abstract

The gap between the current supply and future demand of meat has increased the need to produce plant-based meat analogs. Methylcellulose (MC) is used in most commercial products. Consumers and manufacturers require the development of other novel binding systems, as MC is not chemical-free. We aimed to develop a novel chemical-free binding system for meat analogs. First, we found that laccase (LC) synergistically crosslinks proteins and sugar beet pectin (SBP). To investigate the ability of these SBP-protein crosslinks, textured vegetable protein (TVP) was used. The presence of LC and SBP improved the moldability and binding ability of patties, regardless of the type, shape, and size of TVPs. The hardness of LC-treated patties with SBP reached 32.2 N, which was 1.7- and 7.9-fold higher than that of patties with MC and transglutaminase-treated patties. Additionally, the cooking loss and water/oil-holding capacity of LC-treated patties with SBP improved by up to 8.9-9.4% and 5.8-11.3%, compared with patties with MC. Moreover, after gastrointestinal digestion, free amino nitrogen released from LC-treated patties with SBP was 2.3-fold higher than that released from patties with MC. This is the first study to report protein-SBP crosslinks by LC as chemical-free novel binding systems for meat analogs.

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References

Ismail I, Hwang Y, Joo S . Interventions of two-stage thermal sous-vide cooking on the toughness of beef semitendinosus. Meat Sci. 2019; 157:107882. DOI: 10.1016/j.meatsci.2019.107882. View

Buchert J, Ercili Cura D, Ma H, Gasparetti C, Monogioudi E, Faccio G . Crosslinking food proteins for improved functionality. Annu Rev Food Sci Technol. 2011; 1:113-38. DOI: 10.1146/annurev.food.080708.100841. View

He J, Evans N, Liu H, Shao S . A review of research on plant-based meat alternatives: Driving forces, history, manufacturing, and consumer attitudes. Compr Rev Food Sci Food Saf. 2020; 19(5):2639-2656. DOI: 10.1111/1541-4337.12610. View

Bampidis V, Azimonti G, Bastos M, Christensen H, Dusemund B, Kos Durjava M . Safety and efficacy of methyl cellulose for all animal species. EFSA J. 2020; 18(7):e06212. PMC: 7393478. DOI: 10.2903/j.efsa.2020.6212. View

Miwa N . Innovation in the food industry using microbial transglutaminase: Keys to success and future prospects. Anal Biochem. 2020; 597:113638. DOI: 10.1016/j.ab.2020.113638. View

Heck T, Faccio G, Richter M, Thony-Meyer L . Enzyme-catalyzed protein crosslinking. Appl Microbiol Biotechnol. 2012; 97(2):461-75. PMC: 3546294. DOI: 10.1007/s00253-012-4569-z. View

Fresan U, Sabate J . Vegetarian Diets: Planetary Health and Its Alignment with Human Health. Adv Nutr. 2019; 10(Suppl_4):S380-S388. PMC: 6855976. DOI: 10.1093/advances/nmz019. View

Doi E, Shibata D, Matoba T . Modified colorimetric ninhydrin methods for peptidase assay. Anal Biochem. 1981; 118(1):173-84. DOI: 10.1016/0003-2697(81)90175-5. View

Buhler J, Dekkers B, Bruins M, Jan van der Goot A . Modifying Faba Bean Protein Concentrate Using Dry Heat to Increase Water Holding Capacity. Foods. 2020; 9(8). PMC: 7465143. DOI: 10.3390/foods9081077. View

10.

Oh I, Lee J, Lee H, Lee S . Feasibility of hydroxypropyl methylcellulose oleogel as an animal fat replacer for meat patties. Food Res Int. 2019; 122:566-572. DOI: 10.1016/j.foodres.2019.01.012. View

11.

Lee H, Yong H, Kim M, Choi Y, Jo C . Status of meat alternatives and their potential role in the future meat market - A review. Asian-Australas J Anim Sci. 2020; 33(10):1533-1543. PMC: 7463075. DOI: 10.5713/ajas.20.0419. View

12.

De Angelis D, Kaleda A, Pasqualone A, Vaikma H, Tamm M, Tammik M . Physicochemical and Sensorial Evaluation of Meat Analogues Produced from Dry-Fractionated Pea and Oat Proteins. Foods. 2020; 9(12). PMC: 7760771. DOI: 10.3390/foods9121754. View

13.

Mattinen M, Hellman M, Permi P, Autio K, Kalkkinen N, Buchert J . Effect of protein structure on laccase-catalyzed protein oligomerization. J Agric Food Chem. 2006; 54(23):8883-90. DOI: 10.1021/jf062397h. View

14.

Hassan Kamani M, Meera M, Bhaskar N, Modi V . Partial and total replacement of meat by plant-based proteins in chicken sausage: evaluation of mechanical, physico-chemical and sensory characteristics. J Food Sci Technol. 2019; 56(5):2660-2669. PMC: 6525691. DOI: 10.1007/s13197-019-03754-1. View

15.

Ralet M, Thibault J, Faulds C, Williamson G . Isolation and purification of feruloylated oligosaccharides from cell walls of sugar-beet pulp. Carbohydr Res. 1994; 263(2):227-41. DOI: 10.1016/0008-6215(94)00175-8. View

16.

Takei T, Sugihara K, Yoshida M, Kawakami K . Injectable and biodegradable sugar beet pectin/gelatin hydrogels for biomedical applications. J Biomater Sci Polym Ed. 2013; 24(11):1333-42. DOI: 10.1080/09205063.2012.757727. View

17.

Wi G, Bae J, Kim H, Cho Y, Choi M . Evaluation of the Physicochemical and Structural Properties and the Sensory Characteristics of Meat Analogues Prepared with Various Non-Animal Based Liquid Additives. Foods. 2020; 9(4). PMC: 7230993. DOI: 10.3390/foods9040461. View

18.

Meade S, Reid E, Gerrard J . The impact of processing on the nutritional quality of food proteins. J AOAC Int. 2005; 88(3):904-22. View

19.

Lara-Espinoza C, Carvajal-Millan E, Balandran-Quintana R, Lopez-Franco Y, Rascon-Chu A . Pectin and Pectin-Based Composite Materials: Beyond Food Texture. Molecules. 2018; 23(4). PMC: 6017442. DOI: 10.3390/molecules23040942. View

20.

Zeeb B, Fischer L, Weiss J . Cross-linking of interfacial layers affects the salt and temperature stability of multilayered emulsions consisting of fish gelatin and sugar beet pectin. J Agric Food Chem. 2011; 59(19):10546-55. DOI: 10.1021/jf202220z. View