Live Single-cell Transcriptional Dynamics Via RNA Labelling During the Phosphate Response in Plants

Overview

Journal Nat Plants

Specialties Biology
Genetics

Date 2021 Aug 10

PMID 34373603

Citations 14

Authors

Sahar Hani

Laura Cuyas

Pascale David

David Secco

James Whelan

Marie-Christine Thibaud

Remy Merret

Florian Mueller

Nathalie Pochon

Helene Javot

Orestis Faklaris

Eric Marechal

Edouard Bertrand

Laurent Nussaume

Affiliations

Soon will be listed here.

Abstract

Plants are constantly adapting to ambient fluctuations through spatial and temporal transcriptional responses. Here, we implemented the latest-generation RNA imaging system and combined it with microfluidics to visualize transcriptional regulation in living Arabidopsis plants. This enabled quantitative measurements of the transcriptional activity of single loci in single cells, in real time and under changing environmental conditions. Using phosphate-responsive genes as a model, we found that active genes displayed high transcription initiation rates (one initiation event every ~3 s) and frequently clustered together in endoreplicated cells. We observed gene bursting and large allelic differences in single cells, revealing that at steady state, intrinsic noise dominated extrinsic variations. Moreover, we established that transcriptional repression triggered in roots by phosphate, a crucial macronutrient limiting plant development, occurred with unexpectedly fast kinetics (on the order of minutes) and striking heterogeneity between neighbouring cells. Access to single-cell RNA polymerase II dynamics in live plants will benefit future studies of signalling processes.

Citing Articles

Live Single-Cell Transcriptional Dynamics in Plant Cells.

Hani S, Mercier C, David P, Bertrand E, Desnos T, Nussaume L Methods Mol Biol. 2024; 2875:37-58.

PMID: 39535638 DOI: 10.1007/978-1-0716-4248-1_4.

Sink strength, nutrient allocation, cannabinoid yield, and associated transcript profiles vary in two drug-type Cannabis chemovars.

Jost R, Berkowitz O, Pegg A, Hurgobin B, Tamiru-Oli M, Welling M J Exp Bot. 2024; 76(1):152-174.

PMID: 39225376 PMC: 11659186. DOI: 10.1093/jxb/erae367.

Comparative functional analyses of PHR1, PHL1, and PHL4 transcription factors in regulating Arabidopsis responses to phosphate starvation.

Wang Z, Zheng Z, Liu D Front Plant Sci. 2024; 15:1379562.

PMID: 38708390 PMC: 11066281. DOI: 10.3389/fpls.2024.1379562.

Update of phosphate transport regulations.

Kanno S, Nussaume L J Plant Res. 2024; 137(3):293-295.

PMID: 38700602 DOI: 10.1007/s10265-024-01544-1.

smFISH for Plants.

Hani S, Mercier C, David P, Desnos T, Escudier J, Bertrand E Methods Mol Biol. 2024; 2784:87-100.

PMID: 38502480 DOI: 10.1007/978-1-0716-3766-1_6.

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