Isolation and Characterization of Strain ST9; Rhizomicrobiota and in Planta Studies

Overview

Journal Plants (Basel)

Date 2021 Aug 10

PMID 34371669

Citations 3

Authors

Iris Bertani

Elisa Zampieri

Cristina Bez

Andrea Volante

Vittorio Venturi

Stefano Monaco

Affiliations

Soon will be listed here.

Abstract

The development of biotechnologies based on beneficial microorganisms for improving soil fertility and crop yields could help to address many current agriculture challenges, such as food security, climate change, pest control, soil depletion while decreasing the use of chemical fertilizers and pesticides. Plant growth-promoting (PGP) microbes can be used as probiotics in order to increase plant tolerance/resistance to abiotic/biotic stresses and in this context strains belonging to the group have shown to have potential as PGP candidates. In this study a new isolate is reported and tested for (i) in vitro PGP features, (ii) whole-genome sequence analysis, and (iii) its effects on the rhizosphere microbiota composition, plant growth, and different plant genes expression levels in greenhouse experiments. Results showed that ST9 is an efficient rice root colonizer which integrates into the plant resident-microbiota and affects the expression of several plant genes. The potential use of this strain as a plant probiotic is discussed.

Citing Articles

Herbicide-treated soil as a reservoir of beneficial bacteria: microbiome analysis and PGP bioinoculants in maize.

Galic I, Bez C, Bertani I, Venturi V, Stankovic N Environ Microbiome. 2024; 19(1):107.

PMID: 39695885 PMC: 11657599. DOI: 10.1186/s40793-024-00654-6.

Plant-Growth-Promoting Bacteria.

Bianco C Plants (Basel). 2024; 13(10).

PMID: 38794394 PMC: 11125013. DOI: 10.3390/plants13101323.

A Culturomics-Based Bacterial Synthetic Community for Improving Resilience towards Arsenic and Heavy Metals in the Nutraceutical Plant .

Flores-Duarte N, Pajuelo E, Mateos-Naranjo E, Navarro-Torre S, Rodriguez-Llorente I, Redondo-Gomez S Int J Mol Sci. 2023; 24(8).

PMID: 37108166 PMC: 10138511. DOI: 10.3390/ijms24087003.

References

Callahan B, McMurdie P, Rosen M, Han A, Johnson A, Holmes S . DADA2: High-resolution sample inference from Illumina amplicon data. Nat Methods. 2016; 13(7):581-3. PMC: 4927377. DOI: 10.1038/nmeth.3869. View

Kang B, Yang K, Cho B, Han T, Kim I, Lee M . Production of indole-3-acetic acid in the plant-beneficial strain Pseudomonas chlororaphis O6 is negatively regulated by the global sensor kinase GacS. Curr Microbiol. 2006; 52(6):473-6. DOI: 10.1007/s00284-005-0427-x. View

Ferreira C, Soares H, Soares E . Promising bacterial genera for agricultural practices: An insight on plant growth-promoting properties and microbial safety aspects. Sci Total Environ. 2019; 682:779-799. DOI: 10.1016/j.scitotenv.2019.04.225. View

Hu W, Gao Q, Hamada M, Dawood D, Zheng J, Chen Y . Potential of Pseudomonas chlororaphis subsp. aurantiaca Strain Pcho10 as a Biocontrol Agent Against Fusarium graminearum. Phytopathology. 2014; 104(12):1289-97. DOI: 10.1094/PHYTO-02-14-0049-R. View

Better M, Lewis B, Corbin D, Ditta G, Helinski D . Structural relationships among Rhizobium meliloti symbiotic promoters. Cell. 1983; 35(2 Pt 1):479-85. DOI: 10.1016/0092-8674(83)90181-2. View

Solanki M, Garg F . The use of lacZ marker in enumeration of Azotobacter chroococcum in carrier based inoculants. Braz J Microbiol. 2014; 45(2):595-601. PMC: 4166287. DOI: 10.1590/s1517-83822014000200030. View

Bric J, Bostock R, Silverstone S . Rapid in situ assay for indoleacetic Acid production by bacteria immobilized on a nitrocellulose membrane. Appl Environ Microbiol. 1991; 57(2):535-8. PMC: 182744. DOI: 10.1128/aem.57.2.535-538.1991. View

Peng H, Zhang P, Bilal M, Wang W, Hu H, Zhang X . Enhanced biosynthesis of phenazine-1-carboxamide by engineered Pseudomonas chlororaphis HT66. Microb Cell Fact. 2018; 17(1):117. PMC: 6060551. DOI: 10.1186/s12934-018-0962-3. View

Zhang L, Khabbaz S, Wang A, Li H, Abbasi P . Detection and characterization of broad-spectrum antipathogen activity of novel rhizobacterial isolates and suppression of Fusarium crown and root rot disease of tomato. J Appl Microbiol. 2014; 118(3):685-703. DOI: 10.1111/jam.12728. View

10.

Nam H, Anderson A, Kim Y . Biocontrol Efficacy of Formulated O6 against Plant Diseases and Root-Knot Nematodes. Plant Pathol J. 2018; 34(3):241-249. PMC: 5985650. DOI: 10.5423/PPJ.NT.12.2017.0264. View

11.

Lemoine F, Correia D, Lefort V, Doppelt-Azeroual O, Mareuil F, Cohen-Boulakia S . NGPhylogeny.fr: new generation phylogenetic services for non-specialists. Nucleic Acids Res. 2019; 47(W1):W260-W265. PMC: 6602494. DOI: 10.1093/nar/gkz303. View

12.

Huber B, Riedel K, Hentzer M, Heydorn A, Gotschlich A, Givskov M . The cep quorum-sensing system of Burkholderia cepacia H111 controls biofilm formation and swarming motility. Microbiology (Reading). 2001; 147(Pt 9):2517-2528. DOI: 10.1099/00221287-147-9-2517. View

13.

Zhao L, Xu Y, Ma Z, Deng Z, Shan C, Wei G . Colonization and plant growth promoting characterization of endophytic Pseudomonas chlororaphis strain Zong1 isolated from Sophora alopecuroides root nodules. Braz J Microbiol. 2013; 44(2):623-31. PMC: 3833168. DOI: 10.1590/S1517-83822013000200043. View

14.

Liu H, He Y, Jiang H, Peng H, Huang X, Zhang X . Characterization of a phenazine-producing strain Pseudomonas chlororaphis GP72 with broad-spectrum antifungal activity from green pepper rhizosphere. Curr Microbiol. 2007; 54(4):302-6. DOI: 10.1007/s00284-006-0444-4. View

15.

Mosquito S, Bertani I, Licastro D, Compant S, Myers M, Hinarejos E . In Planta Colonization and Role of T6SS in Two Rice Endophytes. Mol Plant Microbe Interact. 2019; 33(2):349-363. DOI: 10.1094/MPMI-09-19-0256-R. View

16.

Nandi M, Selin C, Brassinga A, Belmonte M, Fernando W, Loewen P . Pyrrolnitrin and Hydrogen Cyanide Production by Pseudomonas chlororaphis Strain PA23 Exhibits Nematicidal and Repellent Activity against Caenorhabditis elegans. PLoS One. 2015; 10(4):e0123184. PMC: 4406715. DOI: 10.1371/journal.pone.0123184. View

17.

Burr S, Gobeli S, Kuhnert P, Goldschmidt-Clermont E, Frey J . Pseudomonas chlororaphis subsp. piscium subsp. nov., isolated from freshwater fish. Int J Syst Evol Microbiol. 2010; 60(Pt 12):2753-2757. DOI: 10.1099/ijs.0.011692-0. View

18.

Pfaffl M, Horgan G, Dempfle L . Relative expression software tool (REST) for group-wise comparison and statistical analysis of relative expression results in real-time PCR. Nucleic Acids Res. 2002; 30(9):e36. PMC: 113859. DOI: 10.1093/nar/30.9.e36. View

19.

Cho S, Chang H, Egamberdieva D, Kamilova F, Lugtenberg B, Kuo C . Genome Analysis of Pseudomonas fluorescens PCL1751: A Rhizobacterium that Controls Root Diseases and Alleviates Salt Stress for Its Plant Host. PLoS One. 2015; 10(10):e0140231. PMC: 4599888. DOI: 10.1371/journal.pone.0140231. View

20.

Smeltzer M, Hart M, Iandolo J . Quantitative spectrophotometric assay for staphylococcal lipase. Appl Environ Microbiol. 1992; 58(9):2815-9. PMC: 183012. DOI: 10.1128/aem.58.9.2815-2819.1992. View