Characterization of a Phenazine-producing Strain Pseudomonas Chlororaphis GP72 with Broad-spectrum Antifungal Activity from Green Pepper Rhizosphere

Overview

Journal Curr Microbiol

Specialty Microbiology

Date 2007 Mar 6

PMID 17334842

Citations 27

Authors

Haiming Liu

Yanjing He

Haixia Jiang

Huasong Peng

Xianqing Huang

Xuehong Zhang

Linda S Thomashow

Yuquan Xu

Affiliations

Soon will be listed here.

Abstract

A new Pseudomonas strain, designated GP72, was isolated from green pepper rhizosphere and identified as a member of species Pseudomonas chlororaphis based on morphology; conventional biochemical and physiologic tests; Biolog GN system (Biolog Inc., Hayward, CA); and 16S rDNA sequence analysis. The secondary metabolites produced by this strain have shown broad-spectrum antifungal activity against various phytopathogens of agricultural importance in vitro. Two main antifungal substances produced by this strain proved to be phenazine-1-carboxylic acid and 2-hydroxyphenazine with further purification and structure elucidation based on ultraviolet-absorbent spectrum scanning, atmospheric pressure chemical ionization-mass spectrometry (APCI-MS) spectrum, and (1)H,(13)C nuclear magnetic resonance spectrums. Strain GP72 could produce quorum-sensing signaling molecules of N-butanoyl-L-homoserine lactone and N-hexanoyl-L-homoserine lactone, which were found to accumulate with different quantities in King's medium B and pigment producing medium, respectively.

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