Siberian Wildrye ( L.) Abscisic Acid-Insensitive 5 Gene Is Involved in Abscisic Acid-Dependent Salt Response

Siberian wildrye ( L.) is a salt-tolerant, high-quality forage grass that plays an important role in forage production and ecological restoration. Abscisic acid (ABA)-insensitive 5 () is essential for the normal functioning of the ABA signal pathway. However, the role of from Siberian wildrye under salt stress remains unclear. Here, we evaluated the role of L. abscisic acid-insensitive 5 () in the ABA-dependent regulation of the response of Siberian wildrye to salt stress. The open reading frame length of isolated from Siberian wildrye was 1170 bp, and it encoded a 389 amino acid protein, which was localized to the nucleus, with obvious coiled coil areas. EsABI5 had high homology, with ABI5 proteins from , , , and . The conserved domains of EsABI5 belonged to the basic leucine zipper domain superfamily. EsABI5 had 10 functional interaction proteins with credibility greater than 0.7. expression was upregulated in roots and leaves under NaCl stress and was upregulated in leaves and downregulated in roots under ABA treatment. Notably, tobacco plants overexpressing the were more sensitive to salt stress, as confirmed by the determining of related physiological indicators. expression affected the ABA and mitogen-activated protein kinase pathways. Therefore, is involved in antisalt responses in these pathways and plays a negative regulatory role during salt stress.