Selection of Suitable Reference Genes for RT-qPCR Gene Expression Analysis in Siberian Wild Rye () Under Different Experimental Conditions

Overview

Journal Genes (Basel)

Publisher MDPI

Date 2019 Jun 16

PMID 31200580

Citations 17

Authors

Junchao Zhang

Wengang Xie

Xinxuan Yu

Zongyu Zhang

Yongqiang Zhao

Na Wang

Yanrong Wang

Affiliations

Soon will be listed here.

Abstract

, which is a perennial and self-pollinated grass, is the typical species of the genus , which plays an important role in forage production and ecological restoration. No reports have, so far, systematically described the selection of optimal reference genes for reverse transcriptase quantitative real-time polymerase chain reaction (RT-qPCR) analysis in . The goals of this study were to evaluate the expression stability of 13 candidate reference genes in different experimental conditions, and to determine the appropriate reference genes for gene expression analysis in . Five methods including Delta Ct (ΔCt), BestKeeper, NormFinder, geNorm, and RefFinder were used to assess the expression stability of 13 potential reference genes. The results of the RefFinder analysis showed that and were the most stable reference genes in different genotypes. and had the most stable expression in different developmental stages. and were suitable reference genes in different tissues. Under salt stress, and were identified as the most stable reference genes. and showed the most stability under heat stress. For cold stress, and presented the highest degree of expression stability. and were considered as the most stable reference genes under osmotic stress. The optimal reference genes were selected to investigate the expression pattern of target gene in different conditions. This study provides suitable reference genes for further gene expression analysis using RT-qPCR in .

Citing Articles

Genome-Wide Exploration and Characterization of the Gene Family's Expression Patterns in Response to Abiotic Stresses in Siberian Wildrye ( L.).

Liu T, Peng J, Dong Z, Liu Y, Wu J, Xiong Y Int J Mol Sci. 2025; 26(5).

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Systematic identification of reference genes for qRT-PCR of A. DC under different experimental conditions and for anthocyanin-related genes studies.

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Selection of Suitable Reference Genes for RT-qPCR Gene Expression Analysis in Centipedegrass under Different Abiotic Stress.

Wang X, Shu X, Su X, Xiong Y, Xiong Y, Chen M Genes (Basel). 2023; 14(10).

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Integrating Full-Length Transcriptome and RNA Sequencing of Siberian Wildrye () to Reveal Molecular Mechanisms in Response to Drought Stress.

Yu Q, Xiong Y, Su X, Xiong Y, Dong Z, Zhao J Plants (Basel). 2023; 12(14).

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