Regulation Network of Colorectal-Cancer-Specific Enhancers in the Progression of Colorectal Cancer

Overview

Journal Int J Mol Sci

Publisher MDPI

Specialties Biochemistry
Chemistry
Molecular Biology

Date 2021 Aug 7

PMID 34361106

Citations 4

Authors

Bohan Chen

Yiping Ma

Jinfang Bi

Wenbin Wang

Anshun He

Guangsong Su

Zhongfang Zhao

Jiandang Shi

Lei Zhang

Affiliations

Soon will be listed here.

Abstract

Enhancers regulate multiple genes via higher-order chromatin structures, and they further affect cancer progression. Epigenetic changes in cancer cells activate several cancer-specific enhancers that are silenced in normal cells. These cancer-specific enhancers are potential therapeutic targets of cancer. However, the functions and regulation networks of colorectal-cancer-specific enhancers are still unknown. In this study, we profile colorectal-cancer-specific enhancers and reveal their regulation network through the analysis of HiChIP data that were derived from a colorectal cancer cell line and Hi-C and RNA-seq data that were derived from tissue samples by in silico analysis and in vitro experiments. Enhancer-promoter loops in colorectal cancer cells containing colorectal-cancer-specific enhancers are involved in more than 50% of the topological associated domains (TADs) changed in colorectal cancer cells compared to normal colon cells. In addition, colorectal-cancer-specific enhancers interact with 152 genes that are significantly and highly expressed in colorectal cancer cells. These colorectal-cancer-specific enhancer target genes include , , , and . We propose that the regulation network of colorectal-cancer-specific enhancers plays an important role in the progression of colorectal cancer.

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