Regular Transient Limb Ischemia Protects Endothelial Function Against Hypercholesterolemic Damage in Rabbits

Overview

Journal Sci Prog

Publisher Sage Publications

Specialty Science

Date 2021 Aug 5

PMID 34351826

Citations 1

Authors

Hongli Wang

Zhinan Zheng

Nanrong Zhang

Yan Zhou

Sanqing Jin

Affiliations

Soon will be listed here.

Abstract

Regular transient limb ischemia (RTLI) can prevent atherosclerosis in hypercholesterolemic rabbits. As endothelial dysfunction is the initial factor leading to atherosclerosis, we investigated the effect of RTLI on endothelial function in hypercholesterolemic rabbits. We randomly allocated 15 New Zealand white rabbits to three groups, five animals per group: the hypercholesterolemic group (Group H), the sham RTLI group (Group S), and the RTLI group (Group L). All rabbits received hypercholesterolemic fodder daily. No intervention was performed on the rabbits in Group H. Rabbits in Group S were kept in hutches, with a deflated cuff applied to their left hind limb for 60 min every day. For rabbits in Group L, RTLI (six cycles of 5-min ischemia and 5-min reperfusion of the left hind limb) was applied once daily for 12 weeks. At the end of week 12, a segment of the abdominal aorta was isolated from each rabbit for in vitro measurement of the endothelium-dependent vasodilation (EDV) response to different concentrations of acetylcholine and the endothelium-independent vasodilation (EIV) response to sodium nitroprusside. The EDV response was significantly higher in Group L than in Groups S and H ( < 0.05), with no significant difference between Groups S and H ( > 0.05). There was no difference in the EIV response among the three groups. RTLI could improve the EDV response, protecting endothelial function against hypercholesterolemic damage.

Citing Articles

Regular transient limb ischemia improves endothelial function and inhibits endothelial cell apoptosis to prevent atherosclerosis in rabbit.

Zhang N, Wen Y, Li J, Zheng W, Jin S BMC Cardiovasc Disord. 2024; 24(1):209.

PMID: 38627625 PMC: 11020181. DOI: 10.1186/s12872-024-03869-0.

References

Bonetti P, Lerman L, Lerman A . Endothelial dysfunction: a marker of atherosclerotic risk. Arterioscler Thromb Vasc Biol. 2003; 23(2):168-75. DOI: 10.1161/01.atv.0000051384.43104.fc. View

McLenachan J, Williams J, Fish R, Ganz P, Selwyn A . Loss of flow-mediated endothelium-dependent dilation occurs early in the development of atherosclerosis. Circulation. 1991; 84(3):1273-8. DOI: 10.1161/01.cir.84.3.1273. View

Forstermann U, Xia N, Li H . Roles of Vascular Oxidative Stress and Nitric Oxide in the Pathogenesis of Atherosclerosis. Circ Res. 2017; 120(4):713-735. DOI: 10.1161/CIRCRESAHA.116.309326. View

Stokes K, Russell J, Jennings M, Alexander J, Granger D . Platelet-associated NAD(P)H oxidase contributes to the thrombogenic phenotype induced by hypercholesterolemia. Free Radic Biol Med. 2007; 43(1):22-30. PMC: 1975956. DOI: 10.1016/j.freeradbiomed.2007.02.027. View

Filgueira F, Lobato N, Nascimento D, Ceravolo G, Giachini F, Lima V . Equilin displays similar endothelium-independent vasodilator potential to 17β-estradiol regardless of lower potential to inhibit calcium entry. Steroids. 2018; 141:46-54. PMC: 6984400. DOI: 10.1016/j.steroids.2018.11.006. View

Taleb S . Inflammation in atherosclerosis. Arch Cardiovasc Dis. 2016; 109(12):708-715. DOI: 10.1016/j.acvd.2016.04.002. View

FURCHGOTT R, Zawadzki J . The obligatory role of endothelial cells in the relaxation of arterial smooth muscle by acetylcholine. Nature. 1980; 288(5789):373-6. DOI: 10.1038/288373a0. View

Loukogeorgakis S, Panagiotidou A, Broadhead M, Donald A, Deanfield J, MacAllister R . Remote ischemic preconditioning provides early and late protection against endothelial ischemia-reperfusion injury in humans: role of the autonomic nervous system. J Am Coll Cardiol. 2005; 46(3):450-6. DOI: 10.1016/j.jacc.2005.04.044. View

Heitzer T, Schlinzig T, Krohn K, Meinertz T, Munzel T . Endothelial dysfunction, oxidative stress, and risk of cardiovascular events in patients with coronary artery disease. Circulation. 2001; 104(22):2673-8. DOI: 10.1161/hc4601.099485. View

10.

Liang Y, Li Y, He F, Liu X, Zhang J . Long-term, regular remote ischemic preconditioning improves endothelial function in patients with coronary heart disease. Braz J Med Biol Res. 2015; 48(6):568-76. PMC: 4470317. DOI: 10.1590/1414-431X20144452. View

11.

Munzel T, Daiber A . Role of endothelial and macrophage tetrahydrobiopterin in development and progression of atherosclerosis: BH4 puzzle solved?. Cardiovasc Res. 2018; 114(10):1310-1312. DOI: 10.1093/cvr/cvy118. View

12.

Lang J, Kim J, Franke W, Vianna L . Seven consecutive days of remote ischaemic preconditioning improves cutaneous vasodilatory capacity in young adults. J Physiol. 2018; 597(3):757-765. PMC: 6355625. DOI: 10.1113/JP277185. View

13.

Ahmadi N, Ruiz-Garcia J, Hajsadeghi F, Azen S, Mack W, Hodis H . Impaired coronary artery distensibility is an endothelium-dependent process and is associated with vulnerable plaque composition. Clin Physiol Funct Imaging. 2014; 36(4):261-8. DOI: 10.1111/cpf.12220. View

14.

Henderson K, Turk J, Rush J, Laughlin M . Endothelial function in coronary arterioles from pigs with early-stage coronary disease induced by high-fat, high-cholesterol diet: effect of exercise. J Appl Physiol (1985). 2004; 97(3):1159-68. DOI: 10.1152/japplphysiol.00261.2004. View

15.

Bar A, Kus K, Manterys A, Proniewski B, Sternak M, Przyborowski K . Vitamin K-MK-7 improves nitric oxide-dependent endothelial function in ApoE/LDLR mice. Vascul Pharmacol. 2019; 122-123:106581. DOI: 10.1016/j.vph.2019.106581. View

16.

Vane J, Anggard E, Botting R . Regulatory functions of the vascular endothelium. N Engl J Med. 1990; 323(1):27-36. DOI: 10.1056/NEJM199007053230106. View

17.

Luca M, Liuni A, McLaughlin K, Gori T, Parker J . Daily ischemic preconditioning provides sustained protection from ischemia-reperfusion induced endothelial dysfunction: a human study. J Am Heart Assoc. 2013; 2(1):e000075. PMC: 3603254. DOI: 10.1161/JAHA.112.000075. View

18.

Kurtel H, Rodrigues S, Yilmaz C, Yildirim A, Granger D . Impaired vasomotor function induced by the combination of hypertension and hypercholesterolemia. J Am Soc Hypertens. 2013; 7(1):14-23. PMC: 3547651. DOI: 10.1016/j.jash.2012.11.005. View

19.

Hobbs D, Goulding M, Nguyen A, Malaver T, Walker C, George T . Acute ingestion of beetroot bread increases endothelium-independent vasodilation and lowers diastolic blood pressure in healthy men: a randomized controlled trial. J Nutr. 2013; 143(9):1399-405. DOI: 10.3945/jn.113.175778. View

20.

Jones H, Hopkins N, Bailey T, Green D, Cable N, Thijssen D . Seven-day remote ischemic preconditioning improves local and systemic endothelial function and microcirculation in healthy humans. Am J Hypertens. 2014; 27(7):918-25. DOI: 10.1093/ajh/hpu004. View