Variants Ala307Ala and Ser680Ser of 307 and 680 FSHr Polymorphisms Negatively Influence on Assisted Reproductive Techniques Outcome and Determine High Probability of Non-pregnancy in Caucasian Patients

Overview

Journal J Assist Reprod Genet

Publisher Springer

Specialties Genetics
Reproductive Medicine

Date 2021 Aug 4

PMID 34346002

Citations 2

Authors

Belen Monge-Ochoa

Luis Montoro

Elisa Gil-Arribas

Julio Montoya

Eduardo Ruiz-Pesini

Manuel J Lopez-Perez

Francisco de Castro

Carmen Diez-Sanchez

Affiliations

Soon will be listed here.

Abstract

Purpose: To determine the influence of different genotypes of Ala307Thr and Asn680Ser FSHr polymorphisms on controlled ovarian stimulation (COS) outcome and pregnancy.

Methods: This study collected blood and physiological and clinical parameters of 517 Caucasian patients (Statistical power ≥ 80%) that underwent COS treatment. Genotypes of Ala307Thr and Asn680Ser polymorphisms were determined using PCR amplification followed by Bsu36I and BsrI digestion, respectively.

Results: Ala307Ala and Ser680Ser genotypes associated to worse parameters of COS outcome (preovulatory follicles P = 0.05, in both), justifying their lower pregnancy rate than Non-Ala307Ala, P = 0.01 and Non-Ser680Ser, P = 0.004, respectively or together, (P = 0.003). Within the Non-Ala307Ala group, Thr307Thr genotype showed higher number of fertilized oocytes (P = 0.04) and embryos (P = 0.01) than Non-Thr307Thr, but no influence on pregnancy rate. Ala307Ala and Ser680Ser patients doubled probability of non-pregnancy than Non-Ala307Ala (odds ratio = 2.0) and Non-Ser680Ser (odds ratio = 2.11), respectively. Ala307Ala and Ser680Ser genotypes tend to appear together (P < 0.0001), which increases the probability of non-pregnancy.

Conclusions: Ala307Ala and Ser680Ser genotypes of 307 and 680 FSHr polymorphisms associate to worse COS outcome than its respective Non-Ala307Ala and Non-Ser680Ser. Within the Non-Ala307Ala genotypes, Thr307Thr, although shows higher Fertilized Oocytes and Embryos, do not influence on pregnancy rate. Ala307Ala and Ser680Ser genotypes double the probability of Non-Pregnancy than their respective Non-Ala307Ala and Non-Ser680Ser genotypes. Furthermore, the strong tendency of these genotypes to appear together worsens the probability of pregnancy in these patients.

Citing Articles

m.4216 T > C polymorphism in JT cluster determines a lower pregnancy rate in response to controlled ovarian stimulation treatment.

Monge-Ochoa B, Montoro L, Montoya J, Ruiz-Pesini E, Lopez-Perez M, de Castro F J Assist Reprod Genet. 2023; 40(3):671-682.

PMID: 36701026 PMC: 10033795. DOI: 10.1007/s10815-023-02721-2.

Impact of Follicle Stimulating Hormone Receptor (FSHR) Polymorphism on the Efficiency of Co-Treatment with Growth Hormone in a Group of Infertile Women from Romania.

Surcel M, Doroftei B, Neamtiu I, Muresan D, Caracostea G, Goidescu I Diagnostics (Basel). 2022; 12(10).

PMID: 36292058 PMC: 9600645. DOI: 10.3390/diagnostics12102371.

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