Expression of Cell Type Incongruent Alpha-cardiac Actin 1 Subunit in Medulloblastoma Reveals a Novel Mechanism for Cancer Cell Survival and Control of Migration

Overview

Journal Neurooncol Adv

Publisher Oxford University Press

Date 2021 Aug 2

PMID 34337410

Citations 1

Authors

Rahul Suresh

Daniel Picard

Rita Lo

Jamie Beaulieu

Marc Remke

Roberto Jose Diaz

Affiliations

Soon will be listed here.

Abstract

Background: Alterations in actin subunit expression have been reported in multiple cancers, but have not been investigated previously in medulloblastoma.

Methods: Bioinformatic analysis of multiple medulloblastoma tumor databases was performed to profile mRNA levels. Western blot was used to verify protein expression in established medulloblastoma cell lines. Immunofluorescence microscopy was performed to assess ACTC1 localization. Stable cell lines with ACTC1 overexpression were generated and shRNA knockdown of ACTC1 was accomplished. We used PARP1 cleavage by Western blot as a marker of apoptosis and cell survival was determined by FACS viability assay and colony formation. Cell migration with overexpression or knockdown of ACTC1 was determined by the scratch assay. Stress fiber length distribution was assessed by fluorescence microscopy.

Results: mRNA expression is highest in SHH and WNT medulloblastoma among all subgroups. ACTC1 protein was confirmed by Western blot in SHH subgroup and Group 3 subgroup cell lines with the lowest expression in Group 3 cells. Microscopy demonstrated ACTC1 co-localization with F-actin. Overexpression of ACTC1 in Group 3 cells abolished the apoptotic response to Aurora kinase B inhibition. Knockdown of ACTC1 in SHH cells and in Myc overexpressing SHH cells induced apoptosis, impaired colony formation, and inhibited migration. Changes in stress fiber length distribution in medulloblastoma cells are induced by alterations in ACTC1 abundance.

Conclusions: Alpha-cardiac actin (ACTC1) is expressed in SHH medulloblastoma. Expression of this protein in medulloblastoma modifies stress fiber composition and functions in promoting resistance to apoptosis induced by mitotic inhibition, enhancing cell survival, and controlling migration.

Citing Articles

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