Extracellular Vesicles from Neurons Promote Neural Induction of Stem Cells Through Cyclin D1

Overview

Journal J Cell Biol

Specialty Cell Biology

Date 2021 Jul 26

PMID 34309628

Citations 17

Authors

Lu Song

Xinran Tian

Randy Schekman

Affiliations

Soon will be listed here.

Abstract

Extracellular vesicles (EVs) are thought to mediate the transport of proteins and RNAs involved in intercellular communication. Here, we show dynamic changes in the buoyant density and abundance of EVs that are secreted by PC12 cells stimulated with nerve growth factor (NGF), N2A cells treated with retinoic acid to induce neural differentiation, and mouse embryonic stem cells (mESCs) differentiated into neuronal cells. EVs secreted from in vitro differentiated cells promote neural induction of mESCs. Cyclin D1 enriched within the EVs derived from differentiated neuronal cells contributes to this induction. EVs purified from cells overexpressing cyclin D1 are more potent in neural induction of mESC cells. Depletion of cyclin D1 from the EVs reduced the neural induction effect. Our results suggest that EVs regulate neural development through sorting of cyclin D1.

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