Soybean and Lupine Addition in Hen Nutrition-Influence on Egg Immunoreactivity

Overview

Journal Molecules

Publisher MDPI

Specialty Biology

Date 2021 Jul 24

PMID 34299594

Authors

Aneta Tomczak

Michalina Misiak

Magdalena Zielinska-Dawidziak

Affiliations

Soon will be listed here.

Abstract

Modifying hen fodder is a common way of changing eggs composition today. However, there is no information on the effect of the source of protein in the fodder replacement on egg allergenicity. This research aimed to detect potential differences in the immunoreactivity and protein composition of eggs from hens fed with fodder containing legume. The aim of the first step of the study was to select the proper solvent for extracting allergenic proteins from hen eggs. Two of them (containing Tween 20 and Triton 100) were selected, based on protein profile and concentration analysis. Egg-white- and egg-yolk-proteins extracts prepared with them were checked for potential differences, using SDS-PAGE electrophoresis, and then the Western-blot method, using sera from children allergic to eggs and soy. Preliminary studies on the influence of fodder composition on the composition of egg proteins suggest that the addition of soy and lupine to fodder modifies the expression of egg proteins. The observed differences in the immunoreactivity of proteins contained in hen egg-white samples do not seem to be as significant as the appearance of protein with a molecular weight of ~13 kDa in the yolk of eggs obtained from soybean-fed hens. This protein may increase the immunoreactivity of eggs for children allergic solely to soy.

References

Laemmli U . Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970; 227(5259):680-5. DOI: 10.1038/227680a0. View

Guerin-Dubiard C, Pasco M, Hietanen A, Quiros del Bosque A, Nau F, Croguennec T . Hen egg white fractionation by ion-exchange chromatography. J Chromatogr A. 2005; 1090(1-2):58-67. DOI: 10.1016/j.chroma.2005.06.083. View

Kato Y, Oozawa E, Matsuda T . Decrease in antigenic and allergenic potentials of ovomucoid by heating in the presence of wheat flour: dependence on wheat variety and intermolecular disulfide bridges. J Agric Food Chem. 2001; 49(8):3661-5. DOI: 10.1021/jf0102766. View

Smith P, Krohn R, Hermanson G, Mallia A, Gartner F, Provenzano M . Measurement of protein using bicinchoninic acid. Anal Biochem. 1985; 150(1):76-85. DOI: 10.1016/0003-2697(85)90442-7. View

Alessandri C, Calvani Jr M, Rosengart L, Madella C . Anaphylaxis to quail egg. Allergy. 2004; 60(1):128-9. DOI: 10.1111/j.1398-9995.2004.00449.x. View

Amponsah A, Nayak B . Evaluation of the efficiency of three extraction conditions for the immunochemical detection of allergenic soy proteins in different food matrices. J Sci Food Agric. 2017; 98(6):2378-2384. DOI: 10.1002/jsfa.8729. View

Poulsen L, Hansen T, Norgaard A, Vestergaard H, Stahl Skov P, Bindslev-Jensen C . Allergens from fish and egg. Allergy. 2001; 56 Suppl 67:39-42. DOI: 10.1034/j.1398-9995.2001.00912.x. View

Longo G, Berti I, Burks A, Krauss B, Barbi E . IgE-mediated food allergy in children. Lancet. 2013; 382(9905):1656-64. DOI: 10.1016/S0140-6736(13)60309-8. View

Peeters K, Nordlee J, Penninks A, Chen L, Goodman R, Bruijnzeel-Koomen C . Lupine allergy: not simply cross-reactivity with peanut or soy. J Allergy Clin Immunol. 2007; 120(3):647-53. DOI: 10.1016/j.jaci.2007.05.032. View

10.

Steinhoff M, Fischer M, Paschke-Kratzin A . Comparison of extraction conditions for milk and hen's egg allergens. Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2011; 28(4):373-83. DOI: 10.1080/19440049.2010.545957. View

11.

Yeung J, Newsome W, Abbott M . Determination of egg proteins in food products by enzyme immunoassay. J AOAC Int. 2000; 83(1):139-43. View

12.

Caubet J, Wang J . Current understanding of egg allergy. Pediatr Clin North Am. 2011; 58(2):427-43, xi. PMC: 3069662. DOI: 10.1016/j.pcl.2011.02.014. View

13.

Toomer O, Sanders E, Vu T, Livingston M, Wall B, Malheiros R . Potential Transfer of Peanut and/or Soy Proteins from Poultry Feed to the Meat and/or Eggs Produced. ACS Omega. 2020; 5(2):1080-1085. PMC: 6977026. DOI: 10.1021/acsomega.9b03218. View