Harmful R-loops Are Prevented Via Different Cell Cycle-specific Mechanisms

Overview

Journal Nat Commun

Specialty Biology

Date 2021 Jul 23

PMID 34294712

Citations 28

Authors

Marta San Martin-Alonso

Maria E Soler-Oliva

Maria Garcia-Rubio

Tatiana Garcia-Muse

Andres Aguilera

Affiliations

Soon will be listed here.

Abstract

Identifying how R-loops are generated is crucial to know how transcription compromises genome integrity. We show by genome-wide analysis of conditional yeast mutants that the THO transcription complex, prevents R-loop formation in G1 and S-phase, whereas the Sen1 DNA-RNA helicase prevents them only in S-phase. Interestingly, damage accumulates asymmetrically downstream of the replication fork in sen1 cells but symmetrically in the hpr1 THO mutant. Our results indicate that: R-loops form co-transcriptionally independently of DNA replication; that THO is a general and cell-cycle independent safeguard against R-loops, and that Sen1, in contrast to previously believed, is an S-phase-specific R-loop resolvase. These conclusions have important implications for the mechanism of R-loop formation and the role of other factors reported to affect on R-loop homeostasis.

Citing Articles

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