A Single Nucleotide Polymorphism Electrochemical Sensor Based on DNA-functionalized Cd-MOFs-74 As Cascade Signal Amplification Probes

Overview

Journal Mikrochim Acta

Specialties Biotechnology
Chemistry

Date 2021 Jul 22

PMID 34291388

Citations 2

Authors

Jia Li Liu

Yu Chan Ma

Tong Yang

Rong Hu

Yun Hui Yang

Affiliations

Soon will be listed here.

Abstract

An ultrasensitive electrochemical sensor has been constructed for the detection of single nucleotide polymorphisms (SNPs) based on DNA-functionalized Cd-MOFs-74 as cascade signal amplification probe under enzyme-free conditions. Interestingly, the introduction of an auxiliary probe did not disturb the detection of SNP targets, but could bind more Cd-MOFs-74 signal elements to enhance the different pulse voltammetry electrochemical signal 2~3 times as compared to sensing system without auxiliary probe, which obviously improves the sensitivity of the proposed sensor. Experimental results taking p53 tumor suppressor gene as SNP model demonstrated that the proposed method can be employed to sensitively and selectively detect target p53 gene fragment with a linear response ranging from 0.01 to 30 pmol/L (detection limit of 6.3 fmol/L) under enzyme-free conditions. Utilizing this strategy, the ultrasensitive SNP electrochemical sensor is a promising tool for the determination of SNPs in biomedicine. Graphical Abstract.

Citing Articles

Generic Platform for the Multiplexed Targeted Electrochemical Detection of Osteoporosis-Associated Single Nucleotide Polymorphisms Using Recombinase Polymerase Solid-Phase Primer Elongation and Ferrocene-Modified Nucleoside Triphosphates.

Ortiz M, Jauset-Rubio M, Trummer O, Foessl I, Kodr D, Acero J ACS Cent Sci. 2023; 9(8):1591-1602.

PMID: 37637735 PMC: 10450878. DOI: 10.1021/acscentsci.3c00243.

Design and Application of Electrochemical Sensors with Metal-Organic Frameworks as the Electrode Materials or Signal Tags.

Chang Y, Lou J, Yang L, Liu M, Xia N, Liu L Nanomaterials (Basel). 2022; 12(18).

PMID: 36145036 PMC: 9506444. DOI: 10.3390/nano12183248.

References

Duan X, Liu L, Feng F, Wang S . Cationic conjugated polymers for optical detection of DNA methylation, lesions, and single nucleotide polymorphisms. Acc Chem Res. 2009; 43(2):260-70. DOI: 10.1021/ar9001813. View

Cheung K, Abendroth J, Nakatsuka N, Zhu B, Yang Y, Andrews A . Detecting DNA and RNA and Differentiating Single-Nucleotide Variations via Field-Effect Transistors. Nano Lett. 2020; 20(8):5982-5990. PMC: 7439785. DOI: 10.1021/acs.nanolett.0c01971. View

Du Y, Dong S . Nucleic Acid Biosensors: Recent Advances and Perspectives. Anal Chem. 2017; 89(1):189-215. DOI: 10.1021/acs.analchem.6b04190. View

Abi A, Safavi A . Targeted Detection of Single-Nucleotide Variations: Progress and Promise. ACS Sens. 2019; 4(4):792-807. DOI: 10.1021/acssensors.8b01604. View

Wang C, Chen C, Jong Y, Kou H . Specific Gene Capture Combined with Restriction-Fragment Release for Directly Fluorescent Genotyping of Single-Nucleotide Polymorphisms in Diagnosing Spinal Muscular Atrophy. Anal Chem. 2018; 90(19):11599-11606. DOI: 10.1021/acs.analchem.8b02996. View

Kausar A, Osman E, Gadzikwa T, Gibbs-Davis J . The presence of a 5'-abasic lesion enhances discrimination of single nucleotide polymorphisms while inducing an isothermal ligase chain reaction. Analyst. 2016; 141(14):4272-7. DOI: 10.1039/c6an00614k. View

Qiu L, Wu Z, Shen G, Yu R . Highly sensitive and selective bifunctional oligonucleotide probe for homogeneous parallel fluorescence detection of protein and nucleotide sequence. Anal Chem. 2011; 83(8):3050-7. DOI: 10.1021/ac103274j. View

Feng Y, Sun F, Wang N, Lei J, Ju H . Ru(bpy) Incorporated Luminescent Polymer Dots: Double-Enhanced Electrochemiluminescence for Detection of Single-Nucleotide Polymorphism. Anal Chem. 2017; 89(14):7659-7666. DOI: 10.1021/acs.analchem.7b01603. View

Gu C, Kong X, Liu X, Gai P, Li F . Enzymatic Biofuel-Cell-Based Self-Powered Biosensor Integrated with DNA Amplification Strategy for Ultrasensitive Detection of Single-Nucleotide Polymorphism. Anal Chem. 2019; 91(13):8697-8704. DOI: 10.1021/acs.analchem.9b02510. View

10.

Zhou H, Long J, Yaghi O . Introduction to metal-organic frameworks. Chem Rev. 2012; 112(2):673-4. DOI: 10.1021/cr300014x. View

11.

Zhang W, Bu A, Ji Q, Min L, Zhao S, Wang Y . p-Directed Incorporation of Phosphonates into MOF-808 via Ligand Exchange: Stability and Adsorption Properties for Uranium. ACS Appl Mater Interfaces. 2019; 11(37):33931-33940. DOI: 10.1021/acsami.9b10920. View

12.

He K, Li Z, Wang L, Fu Y, Quan H, Li Y . A Water-Stable Luminescent Metal-Organic Framework for Rapid and Visible Sensing of Organophosphorus Pesticides. ACS Appl Mater Interfaces. 2019; 11(29):26250-26260. DOI: 10.1021/acsami.9b06151. View

13.

Li X, Yang X, Sha J, Han T, Du C, Sun Y . POMOF/SWNT Nanocomposites with Prominent Peroxidase-Mimicking Activity for l-Cysteine "On-Off Switch" Colorimetric Biosensing. ACS Appl Mater Interfaces. 2019; 11(18):16896-16904. DOI: 10.1021/acsami.9b00872. View

14.

Zhang G, Shan D, Dong H, Cosnier S, Al-Ghanim K, Ahmad Z . DNA-Mediated Nanoscale Metal-Organic Frameworks for Ultrasensitive Photoelectrochemical Enzyme-Free Immunoassay. Anal Chem. 2018; 90(20):12284-12291. DOI: 10.1021/acs.analchem.8b03762. View

15.

Valekar A, Batule B, Kim M, Cho K, Hong D, Lee U . Novel amine-functionalized iron trimesates with enhanced peroxidase-like activity and their applications for the fluorescent assay of choline and acetylcholine. Biosens Bioelectron. 2017; 100:161-168. DOI: 10.1016/j.bios.2017.08.056. View

16.

Jiang Z, Gao P, Yang L, Huang C, Li Y . Facile in Situ Synthesis of Silver Nanoparticles on the Surface of Metal-Organic Framework for Ultrasensitive Surface-Enhanced Raman Scattering Detection of Dopamine. Anal Chem. 2015; 87(24):12177-82. DOI: 10.1021/acs.analchem.5b03058. View

17.

Lu M, Deng Y, Luo Y, Lv J, Li T, Xu J . Graphene Aerogel-Metal-Organic Framework-Based Electrochemical Method for Simultaneous Detection of Multiple Heavy-Metal Ions. Anal Chem. 2018; 91(1):888-895. DOI: 10.1021/acs.analchem.8b03764. View

18.

Chang J, Wang X, Wang J, Li H, Li F . Nucleic Acid-Functionalized Metal-Organic Framework-Based Homogeneous Electrochemical Biosensor for Simultaneous Detection of Multiple Tumor Biomarkers. Anal Chem. 2019; 91(5):3604-3610. DOI: 10.1021/acs.analchem.8b05599. View

19.

Liu T, Hu R, Zhang X, Zhang K, Liu Y, Zhang X . Metal-Organic Framework Nanomaterials as Novel Signal Probes for Electron Transfer Mediated Ultrasensitive Electrochemical Immunoassay. Anal Chem. 2017; 88(24):12516-12523. DOI: 10.1021/acs.analchem.6b04191. View

20.

Xie F, Zhao X, Chi K, Yang T, Hu R, Yang Y . Fe-MOFs as signal probes coupling with DNA tetrahedral nanostructures for construction of ratiometric electrochemical aptasensor. Anal Chim Acta. 2020; 1135:123-131. DOI: 10.1016/j.aca.2020.08.007. View