Coupled Sterol Synthesis and Transport Machineries at ER-endocytic Contact Sites

Overview

Journal J Cell Biol

Specialty Cell Biology

Date 2021 Jul 20

PMID 34283201

Citations 13

Authors

Javier Encinar Del Dedo

Isabel Maria Fernandez-Golbano

Laura Pastor

Paula Meler

Cristina Ferrer-Orta

Elena Rebollo

Maria Isabel Geli

Affiliations

Soon will be listed here.

Abstract

Sterols are unevenly distributed within cellular membranes. How their biosynthetic and transport machineries are organized to generate heterogeneity is largely unknown. We previously showed that the yeast sterol transporter Osh2 is recruited to endoplasmic reticulum (ER)-endocytic contacts to facilitate actin polymerization. We now find that a subset of sterol biosynthetic enzymes also localizes at these contacts and interacts with Osh2 and the endocytic machinery. Following the sterol dynamics, we show that Osh2 extracts sterols from these subdomains, which we name ERSESs (ER sterol exit sites). Further, we demonstrate that coupling of the sterol synthesis and transport machineries is required for endocytosis in mother cells, but not in daughters, where plasma membrane loading with accessible sterols and endocytosis are linked to secretion.

Citing Articles

Use of the D4H Probe to Track Sterols in Yeast.

Fernandez-Golbano I, Garcia P, Rebollo E, Geli M, Encinar Del Dedo J Methods Mol Biol. 2024; 2888:35-52.

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ER-associated VAP27-1 and VAP27-3 proteins functionally link the lipid-binding ORP2A at the ER-chloroplast contact sites.

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The molecular mechanism of on-demand sterol biosynthesis at organelle contact sites.

Zung N, Aravindan N, Boshnakovska A, Valenti R, Preminger N, Jonas F bioRxiv. 2024; .

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