Generation of Plasma Cells and CD27IgD B Cells During Hantavirus Infection is Associated with Distinct Pathological Findings

Overview

Journal Clin Transl Immunology

Specialty Allergy & Immunology

Date 2021 Jul 19

PMID 34277007

Citations 8

Authors

Priscilla F Kerkman

Andy Dernstedt

Lalitha Tadala

Eva Mittler

Mirjam Dannborg

Christopher Sundling

Kimia T Maleki

Johanna Tauriainen

Anne Tuiskunen-Back

Julia Wigren Bystrom

Pauline Ocaya

Therese Thunberg

Rohit K Jangra

Gleyder Roman-Sosa

Pablo Guardado-Calvo

Felix A Rey

Jonas Klingstrom

Kartik Chandran

Andrea Puhar

Clas Ahlm

Mattias Ne Forsell

Affiliations

Soon will be listed here.

Abstract

Objective: Human hantavirus infections can cause haemorrhagic fever with renal syndrome (HFRS). The pathogenic mechanisms are not fully understood, nor if they affect the humoral immune system. The objective of this study was to investigate humoral immune responses to hantavirus infection and to correlate them to the typical features of HFRS: thrombocytopenia and transient kidney dysfunction.

Methods: We performed a comprehensive characterisation of longitudinal antiviral B-cell responses of 26 hantavirus patients and combined this with paired clinical data. In addition, we measured extracellular adenosine triphosphate (ATP) and its breakdown products in circulation and performed stimulations to address its effect on B cells.

Results: We found that thrombocytopenia was correlated to an elevated frequency of plasmablasts in circulation. In contrast, kidney dysfunction was indicative of an accumulation of CD27IgD B cells and CD27 plasmablasts. Finally, we provide evidence that high levels of extracellular ATP and matrix metalloproteinase 8 can contribute to shedding of CD27 during human hantavirus infection.

Conclusion: Our findings demonstrate that thrombocytopenia and kidney dysfunction associate with distinctly different effects on the humoral immune system. Moreover, hantavirus-infected individuals have significantly elevated levels of extracellular ATP in circulation.

Citing Articles

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Innate lymphoid cells are activated in HFRS, and their function can be modulated by hantavirus-induced type I interferons.

Garcia M, Carrasco Garcia A, Weigel W, Christ W, Lira-Junior R, Wirth L PLoS Pathog. 2024; 20(7):e1012390.

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Mittler E, Serris A, Esterman E, Florez C, Polanco L, OBrien C Sci Transl Med. 2023; 15(700):eadg1855.

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