Osteogenic Effects of MicroRNA-335-5p/lipidoid Nanoparticles Coated on Titanium Surface

Overview

Journal Arch Oral Biol

Specialty Dentistry

Date 2021 Jul 17

PMID 34273868

Citations 3

Authors

Qiang Wang

Xiaoying Wang

Paloma Valverde

Dana Murray

Michel M Dard

Thomas Van Dyke

Qiaobing Xu

Xin Xu

Nadeem Karimbux

Qisheng Tu

Jake Chen

Affiliations

Soon will be listed here.

Abstract

Objective: In this study, we aimed to investigate the therapeutic potential of miR-335-5p lipidoid nanocomplexes coated on Titanium (Ti) SLActive surface by lyophilization.

Design: In our model, we coated miR-335-5p/Lipidoid nanoparticles on titanium implant, seeded GFP-labelled mouse bone marrow stromal cells (BMSCs) onto the functionalized Ti implant surface, and analyzed the transfection efficiency, cell adhesion, proliferation, and osteogenic activity of the bone-implant interface.

Results: The Ti SLActive surface displayed a suitable hydrophilicity ability and provided a large surface area for miRNA loading, enabling spatial retention of the miRNAs within the nanopores until cellular delivery. We demonstrated a high transfection efficiency of miR-335-5p lipidoid nanoparticles in BMSCs seeded onto the Ti SLActive surface, even after 14 days. Alkaline phosphatase (ALP) activity and cell vitality were significantly increased in BMSCs transfected with miR-335-5p at 7 and 14 days as opposed to cells transfected with negative controls. When miR-335-5p transfected BMSCs were induced to undergo osteogenic differentiation, we detected increased mRNA expression of osteogenic markers including Alkaline phosphatase (ALP), collagen I (COL1), osteocalcin (OCN) and bone sialoprotein (BSP) at 7 and 14 days as compared with negative controls.

Conclusion: MiR-335-5p lipidoid nanoparticles could be used as a new cost-effective methodology to increase the osteogenic capacity of biomedical Ti implants.

Citing Articles

Bone Marrow Stromal Cells Generate a Pro-Healing Inflammasome When Cultured on Titanium-Aluminum-Vanadium Surfaces with Microscale/Nanoscale Structural Features.

Cohen D, Van Duyn C, Deng J, Lodi M, Gallagher M, Sugar J Biomimetics (Basel). 2025; 10(1).

PMID: 39851782 PMC: 11759188. DOI: 10.3390/biomimetics10010066.

RNA Coating Promotes Peri-Implant Osseointegration.

Zhang X, Chen Y, Zhou S, Liu Y, Zhu S, Jia X ACS Biomater Sci Eng. 2024; 10(11):7030-7042.

PMID: 38943625 PMC: 11558559. DOI: 10.1021/acsbiomaterials.4c00133.

Surface-modified titanium and titanium-based alloys for improved osteogenesis: A critical review.

Li J, Zheng Y, Yu Z, Kankala R, Lin Q, Shi J Heliyon. 2024; 10(1):e23779.

PMID: 38223705 PMC: 10784177. DOI: 10.1016/j.heliyon.2023.e23779.

Tailoring combinatorial lipid nanoparticles for intracellular delivery of nucleic acids, proteins, and drugs.

Li Y, Ye Z, Yang H, Xu Q Acta Pharm Sin B. 2022; 12(6):2624-2639.

PMID: 35755280 PMC: 9214058. DOI: 10.1016/j.apsb.2022.04.013.

References

Menini M, Pesce P, Baldi D, Vargas G, Pera P, Izzotti A . Prediction of Titanium Implant Success by Analysis of microRNA Expression in Peri-Implant Tissue. A 5-Year Follow-Up Study. J Clin Med. 2019; 8(6). PMC: 6617176. DOI: 10.3390/jcm8060888. View

Wennerberg A, Galli S, Albrektsson T . Current knowledge about the hydrophilic and nanostructured SLActive surface. Clin Cosmet Investig Dent. 2013; 3:59-67. PMC: 3652359. DOI: 10.2147/CCIDEN.S15949. View

Kretlow J, Jin Y, Liu W, Zhang W, Hong T, Zhou G . Donor age and cell passage affects differentiation potential of murine bone marrow-derived stem cells. BMC Cell Biol. 2008; 9:60. PMC: 2584028. DOI: 10.1186/1471-2121-9-60. View

De Laporte L, Shea L . Matrices and scaffolds for DNA delivery in tissue engineering. Adv Drug Deliv Rev. 2007; 59(4-5):292-307. PMC: 1949490. DOI: 10.1016/j.addr.2007.03.017. View

Beutner R, Michael J, Schwenzer B, Scharnweber D . Biological nano-functionalization of titanium-based biomaterial surfaces: a flexible toolbox. J R Soc Interface. 2009; 7 Suppl 1:S93-S105. PMC: 2843991. DOI: 10.1098/rsif.2009.0418.focus. View

Menini M, Dellepiane E, Baldi D, Longobardi M, Pera P, Izzotti A . Microarray expression in peri-implant tissue next to different titanium implant surfaces predicts clinical outcomes: a split-mouth study. Clin Oral Implants Res. 2016; 28(9):e121-e134. DOI: 10.1111/clr.12943. View

Sun S, Wang M, Knupp S, Soto-Feliciano Y, Hu X, Kaplan D . Combinatorial library of lipidoids for in vitro DNA delivery. Bioconjug Chem. 2011; 23(1):135-40. PMC: 3261308. DOI: 10.1021/bc200572w. View

Hu H, Hilton M, Tu X, Yu K, Ornitz D, Long F . Sequential roles of Hedgehog and Wnt signaling in osteoblast development. Development. 2004; 132(1):49-60. DOI: 10.1242/dev.01564. View

Jewell C, Lynn D . Surface-Mediated Delivery of DNA: Cationic Polymers Take Charge. Curr Opin Colloid Interface Sci. 2009; 13(6):395-402. PMC: 2621072. DOI: 10.1016/j.cocis.2008.03.005. View

10.

Akinc A, Goldberg M, Qin J, Dorkin J, Gamba-Vitalo C, Maier M . Development of lipidoid-siRNA formulations for systemic delivery to the liver. Mol Ther. 2009; 17(5):872-9. PMC: 2835134. DOI: 10.1038/mt.2009.36. View

11.

Akinc A, Zumbuehl A, Goldberg M, Leshchiner E, Busini V, Hossain N . A combinatorial library of lipid-like materials for delivery of RNAi therapeutics. Nat Biotechnol. 2008; 26(5):561-9. PMC: 3014085. DOI: 10.1038/nbt1402. View

12.

Jang J, Rives C, Shea L . Plasmid delivery in vivo from porous tissue-engineering scaffolds: transgene expression and cellular transfection. Mol Ther. 2005; 12(3):475-83. PMC: 2648405. DOI: 10.1016/j.ymthe.2005.03.036. View

13.

Monaghan M, Pandit A . RNA interference therapy via functionalized scaffolds. Adv Drug Deliv Rev. 2011; 63(4-5):197-208. DOI: 10.1016/j.addr.2011.01.006. View

14.

Jayawardena T, Egemnazarov B, Finch E, Zhang L, Payne J, Pandya K . MicroRNA-mediated in vitro and in vivo direct reprogramming of cardiac fibroblasts to cardiomyocytes. Circ Res. 2012; 110(11):1465-73. PMC: 3380624. DOI: 10.1161/CIRCRESAHA.112.269035. View

15.

Sui L, Wang M, Han Q, Yu L, Zhang L, Zheng L . A novel Lipidoid-MicroRNA formulation promotes calvarial bone regeneration. Biomaterials. 2018; 177:88-97. PMC: 6019203. DOI: 10.1016/j.biomaterials.2018.05.038. View

16.

Asaad F, Monje A, Larsson L . Role of epigenetics in alveolar bone resorption and regeneration around periodontal and peri-implant tissues. Eur J Oral Sci. 2019; 127(6):477-493. DOI: 10.1111/eos.12657. View

17.

Kern B, Shen J, Starbuck M, Karsenty G . Cbfa1 contributes to the osteoblast-specific expression of type I collagen genes. J Biol Chem. 2000; 276(10):7101-7. DOI: 10.1074/jbc.M006215200. View

18.

Buser D, Broggini N, Wieland M, Schenk R, Denzer A, Cochran D . Enhanced bone apposition to a chemically modified SLA titanium surface. J Dent Res. 2004; 83(7):529-33. DOI: 10.1177/154405910408300704. View

19.

Tome M, Lopez-Romero P, Albo C, Sepulveda J, Fernandez-Gutierrez B, Dopazo A . miR-335 orchestrates cell proliferation, migration and differentiation in human mesenchymal stem cells. Cell Death Differ. 2010; 18(6):985-95. PMC: 3131940. DOI: 10.1038/cdd.2010.167. View

20.

Park J, Jang J, Lee C, Hanawa T . Osteoconductivity of hydrophilic microstructured titanium implants with phosphate ion chemistry. Acta Biomater. 2009; 5(6):2311-21. DOI: 10.1016/j.actbio.2009.02.026. View