Latent Tri-lineage Potential of Human Menstrual Blood-Derived Mesenchymal Stromal Cells Revealed by Specific In Vitro Culture Conditions

Overview

Journal Mol Neurobiol

Specialties Molecular Biology
Neurology

Date 2021 Jul 16

PMID 34269964

Citations 7

Authors

Diana Quintero-Espinosa

Viviana Soto-Mercado

Catherine Quintero-Quinchia

Miguel Mendivil-Perez

Carlos Velez-Pardo

Marlene Jimenez-Del-Rio

Affiliations

Soon will be listed here.

Abstract

Human menstrual blood-derived mesenchymal stromal cells (MenSCs) have become not only an important source of stromal cells for cell therapy but also a cellular source for neurologic disorders in vitro modeling. By using culture protocols originally developed in our laboratory, we show that MenSCs can be converted into floating neurospheres (NSs) using the Fast-N-Spheres medium for 24-72 h and can be transdifferentiated into functional dopaminergic-like (DALNs, ~ 26% TH + /DAT + flow cytometry) and cholinergic-like neurons (ChLNs, ~ 46% ChAT + /VAChT flow cytometry) which responded to dopamine- and acetylcholine-triggered neuronal Ca inward stimuli when cultured with the NeuroForsk and the Cholinergic-N-Run medium, respectively in a timely fashion (i.e., 4-7 days). Here, we also report a direct transdifferentiation method to induce MenSCs into functional astrocyte-like cells (ALCs) by incubation of MenSCs in commercial Gibco® Astrocyte medium in 7 days. The MSC-derived ALCs (~ 59% GFAP + /S100β +) were found to respond to glutamate-induced Ca inward stimuli. Altogether, these results show that MenSCs are a reliable source to obtain functional neurogenic cells to further investigate the neurobiology of neurologic disorders.

Citing Articles

Combination of Tramiprosate, Curcumin, and SP600125 Reduces the Neuropathological Phenotype in Familial Alzheimer Disease PSEN1 I416T Cholinergic-like Neurons.

Gomez-Sequeda N, Jimenez-Del-Rio M, Velez-Pardo C Int J Mol Sci. 2024; 25(9).

PMID: 38732141 PMC: 11084854. DOI: 10.3390/ijms25094925.

High Yield of Functional Dopamine-like Neurons Obtained in NeuroForsk 2.0 Medium to Study Acute and Chronic Rotenone Effects on Oxidative Stress, Autophagy, and Apoptosis.

Quintero-Espinosa D, Velez-Pardo C, Jimenez-Del-Rio M Int J Mol Sci. 2023; 24(21).

PMID: 37958728 PMC: 10647258. DOI: 10.3390/ijms242115744.

Cholinergic-like neurons and cerebral spheroids bearing the PSEN1 p.Ile416Thr variant mirror Alzheimer's disease neuropathology.

Gomez-Sequeda N, Mendivil-Perez M, Jimenez-Del-Rio M, Lopera F, Velez-Pardo C Sci Rep. 2023; 13(1):12833.

PMID: 37553376 PMC: 10409854. DOI: 10.1038/s41598-023-39630-4.

Body fluid-derived stem cells - an untapped stem cell source in genitourinary regeneration.

Huang R, Li Q, Ma J, Atala A, Zhang Y Nat Rev Urol. 2023; 20(12):739-761.

PMID: 37414959 PMC: 11639537. DOI: 10.1038/s41585-023-00787-2.

PSEN1 E280A Cholinergic-like Neurons and Cerebral Spheroids Derived from Mesenchymal Stromal Cells and from Induced Pluripotent Stem Cells Are Neuropathologically Equivalent.

Mendivil-Perez M, Velez-Pardo C, Lopera F, Kosik K, Jimenez-Del-Rio M Int J Mol Sci. 2023; 24(10).

PMID: 37240306 PMC: 10218810. DOI: 10.3390/ijms24108957.

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