Hierarchical Cluster Analysis in the Study of the Effect of Cytokine Expression Patterns on Endometrial Repair and Receptivity After Hysteroscopic Adhesiolysis

Overview

Journal Ann Transl Med

Publisher AME Publishing Company

Date 2021 Jul 16

PMID 34268359

Citations 1

Authors

Bohan Li

Hua Duan

Sha Wang

Yiyi Wang

Yanan Chang

Zhengchen Guo

Yazhu Li

Affiliations

Soon will be listed here.

Abstract

Background: In a previous study, we reported that amnion promotes endometrial cell growth by regulating cytokines. In this study, hierarchical cluster analysis enabled the evaluation of cytokine expression changes after amnion treatment to be explored by cluster patterns. The role of on endometrial repair and receptivity was revealed.

Methods: A total of 30 patients were recruited in this clinical trial (NCT02496052) of hysteroscopic adhesiolysis. They were randomly allocated into an amnion grafts group (amnion group) and a control group. After hysteroscopic adhesiolysis, a Foley catheter covered with a sterilized freeze-dried amnion graft was inserted into the uterine cavity of the participants in the amnion group, whereas for the control group, a Foley catheter without amnion graft was inserted. After surgery, patient follow-up was done for a year. Uterine exudates were collected every day for seven days after surgery, and analyzed by enzyme-linked immunosorbent assays. Hierarchical cluster analysis was performed to compare expression patterns of each cytokine. Single-gene gene set enrichment analysis and differentially expressed genes enrichment analysis of were performed using NCBI GEO (N=151) to evaluate its potential mechanisms and impact on endometrial receptivity.

Results: Compared to the control group, cytokine expression patterns of the amnion group revealed significant stratifications, which were highly correlated with the expression levels of on the sixth to seventh day after surgery, improving the pregnant rate. Wilcoxon test revealed significantly low expression levels of in the reduced endometrial receptivity group compared to the normal group. Moreover, gene set enrichment analysis showed that lysosomes, cell cycle, and calcium signaling pathways were associated with the biological processes in which plays a role. Screening and enrichment analyses of differentially expressed genes further verified the mechanisms of action of on endometrial repair and receptivity recovery.

Conclusions: Amnion promotes endometrial repair and receptivity by altering the expression levels and patterns of . Furthermore, by affecting lysosomal, cell cycle, and calcium signaling pathways, may be one of the factors involved in endometrial repair and receptivity recovery.

Citing Articles

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References

Rossi M, Sharkey A, Vigano P, Fiore G, Furlong R, Florio P . Identification of genes regulated by interleukin-1beta in human endometrial stromal cells. Reproduction. 2005; 130(5):721-9. DOI: 10.1530/rep.1.00688. View

Claude-Taupin A, Bissa B, Jia J, Gu Y, Deretic V . Role of autophagy in IL-1β export and release from cells. Semin Cell Dev Biol. 2018; 83:36-41. PMC: 6173661. DOI: 10.1016/j.semcdb.2018.03.012. View

Konci R, Caminsky N, Tulandi T, Dahan M . Supplements to Conventional Treatment After Hysteroscopic Lysis of Intrauterine Adhesions: A Systematic Review. J Obstet Gynaecol Can. 2019; 42(8):984-1000. DOI: 10.1016/j.jogc.2019.09.008. View

. AAGL Practice Report: Practice Guidelines on Intrauterine Adhesions Developed in Collaboration With the European Society of Gynaecological Endoscopy (ESGE). J Minim Invasive Gynecol. 2017; 24(5):695-705. DOI: 10.1016/j.jmig.2016.11.008. View

Gan L, Duan H, Sun F, Xu Q, Tang Y, Wang S . Efficacy of freeze-dried amnion graft following hysteroscopic adhesiolysis of severe intrauterine adhesions. Int J Gynaecol Obstet. 2017; 137(2):116-122. DOI: 10.1002/ijgo.12112. View

Rebe C, Ghiringhelli F . Interleukin-1β and Cancer. Cancers (Basel). 2020; 12(7). PMC: 7408158. DOI: 10.3390/cancers12071791. View

Koot Y, van Hooff S, Boomsma C, van Leenen D, Groot Koerkamp M, Goddijn M . An endometrial gene expression signature accurately predicts recurrent implantation failure after IVF. Sci Rep. 2016; 6:19411. PMC: 4726345. DOI: 10.1038/srep19411. View

Jirsova K, Jones G . Amniotic membrane in ophthalmology: properties, preparation, storage and indications for grafting-a review. Cell Tissue Bank. 2017; 18(2):193-204. DOI: 10.1007/s10561-017-9618-5. View

Shakya K, Ruskin H, Kerr G, Crane M, Becker J . Comparison of microarray preprocessing methods. Adv Exp Med Biol. 2010; 680:139-47. DOI: 10.1007/978-1-4419-5913-3_16. View

10.

Pathare A, Zaveri K, Hinduja I . Downregulation of genes related to immune and inflammatory response in IVF implantation failure cases under controlled ovarian stimulation. Am J Reprod Immunol. 2017; 78(1). DOI: 10.1111/aji.12679. View

11.

Abudukeyoumu A, Li M, Xie F . Transforming growth factor-β1 in intrauterine adhesion. Am J Reprod Immunol. 2020; 84(2):e13262. DOI: 10.1111/aji.13262. View

12.

. Database resources of the National Center for Biotechnology Information. Nucleic Acids Res. 2017; 46(D1):D8-D13. PMC: 5753372. DOI: 10.1093/nar/gkx1095. View

13.

Zhang S, Li P, Yuan Z, Tan J . Effects of platelet-rich plasma on the activity of human menstrual blood-derived stromal cells in vitro. Stem Cell Res Ther. 2018; 9(1):48. PMC: 6389087. DOI: 10.1186/s13287-018-0795-3. View

14.

Lee Y, Wysocki A, Warburton D, Tuan T . Wound healing in development. Birth Defects Res C Embryo Today. 2012; 96(3):213-22. PMC: 3678537. DOI: 10.1002/bdrc.21017. View

15.

Yu G, Wang L, Han Y, He Q . clusterProfiler: an R package for comparing biological themes among gene clusters. OMICS. 2012; 16(5):284-7. PMC: 3339379. DOI: 10.1089/omi.2011.0118. View

16.

Xin L, Lin X, Zhou F, Li C, Wang X, Yu H . A scaffold laden with mesenchymal stem cell-derived exosomes for promoting endometrium regeneration and fertility restoration through macrophage immunomodulation. Acta Biomater. 2020; 113:252-266. DOI: 10.1016/j.actbio.2020.06.029. View

17.

Gautier L, Cope L, Bolstad B, Irizarry R . affy--analysis of Affymetrix GeneChip data at the probe level. Bioinformatics. 2004; 20(3):307-15. DOI: 10.1093/bioinformatics/btg405. View

18.

Johary J, Xue M, Zhu X, Xu D, Velu P . Efficacy of estrogen therapy in patients with intrauterine adhesions: systematic review. J Minim Invasive Gynecol. 2013; 21(1):44-54. DOI: 10.1016/j.jmig.2013.07.018. View

19.

Wang H, Shi G, Li M, Fan H, Ma H, Sheng L . Correlation of IL-1 and HB-EGF with endometrial receptivity. Exp Ther Med. 2018; 16(6):5130-5136. PMC: 6257333. DOI: 10.3892/etm.2018.6840. View

20.

Peng X, Li T, Zhao Y, Guo Y, Xia E . Safety and Efficacy of Amnion Graft in Preventing Reformation of Intrauterine Adhesions. J Minim Invasive Gynecol. 2017; 24(7):1204-1210. DOI: 10.1016/j.jmig.2017.08.005. View