Fluorescence-Detected Mid-Infrared Photothermal Microscopy

Overview

Journal J Am Chem Soc

Publisher American Chemical Society

Specialty Chemistry

Date 2021 Jul 15

PMID 34264654

Citations 23

Authors

Yi Zhang

Haonan Zong

Cheng Zong

Yuying Tan

Meng Zhang

Yuewei Zhan

Ji-Xin Cheng

Affiliations

Soon will be listed here.

Abstract

Mid-infrared photothermal microscopy is a new chemical imaging technology in which a visible beam senses the photothermal effect induced by a pulsed infrared laser. This technology provides infrared spectroscopic information at submicrometer spatial resolution and enables infrared spectroscopy and imaging of living cells and organisms. Yet, current mid-infrared photothermal imaging sensitivity suffers from a weak dependence of scattering on the temperature, and the image quality is vulnerable to the speckles caused by scattering. Here, we present a novel version of mid-infrared photothermal microscopy in which thermosensitive fluorescent probes are harnessed to sense the mid-infrared photothermal effect. The fluorescence intensity can be modulated at the level of 1% per Kelvin, which is 100 times larger than the modulation of scattering intensity. In addition, fluorescence emission is free of interference, thus much improving the image quality. Moreover, fluorophores can target specific organelles or biomolecules, thus augmenting the specificity of photothermal imaging. Spectral fidelity is confirmed through fingerprinting a single bacterium. Finally, the photobleaching issue is successfully addressed through the development of a wide-field fluorescence-detected mid-infrared photothermal microscope which allows video rate bond-selective imaging of biological specimens.

Citing Articles

Mid-Infrared Photonic Sensors: Exploring Fundamentals, Advanced Materials, and Cutting-Edge Applications.

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INSPIRE: Single-beam probed complementary vibrational bioimaging.

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A tutorial on optical photothermal infrared (O-PTIR) microscopy.

Prater C, Kansiz M, Cheng J APL Photonics. 2024; 9(9):091101.

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Roadmap on Label-Free Super-Resolution Imaging.

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Structural Mapping of Protein Aggregates in Live Cells Modeling Huntington's Disease.

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