A Semiautomated Luciferase Immunoprecipitation Assay for Rapid and Easy Detection of African Swine Fever Virus Antibody

Overview

Journal J Clin Microbiol

Specialty Microbiology

Date 2021 Jul 14

PMID 34260273

Citations 10

Authors

Huan Liu

Ping He

Fei Meng

Mengwei Jiang

Jin Xiong

Junhua Li

Junping Yu

Hongping Wei

Affiliations

Soon will be listed here.

Abstract

African swine fever (ASF) is a highly contagious viral disease of domestic pigs and wild boars. For disease surveillance and control, we developed a rapid and easy luciferase immunoprecipitation assay (MB-LIPS) to detect ASF virus (ASFV) antibody. The MB-LIPS is based on magnetic beads modified with protein A/G and the recombinant fusion protein of ASFV p30 and luciferase, where p30 functioned as the recognition element and luciferase as the signal component. Incubation and washing could be finished automatically on a machine with magnetic rods. Under optimal conditions, the MB-LIPS showed 96.3% agreement to a commercial enzyme-linked immunosorbent assay (ELISA) kit for detecting ASFV antibody in swine sera. Analyzing serial dilutions of a swine serum sample showed that the MP-LIPS assay was 4 times more sensitive than the ELISA kit. The whole run of the MB-LIPS could be completed within 30 min. With its high sensitivity and simple operation, the MB-LIPS platform has great potential to be used for the detection of ASFV antibody and ASF control in small labs and farms.

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References

Perez-Filgueira D, Gonzalez-Camacho F, Gallardo C, Resino-Talavan P, Blanco E, Gomez-Casado E . Optimization and validation of recombinant serological tests for African Swine Fever diagnosis based on detection of the p30 protein produced in Trichoplusia ni larvae. J Clin Microbiol. 2006; 44(9):3114-21. PMC: 1594705. DOI: 10.1128/JCM.00406-06. View

Teklue T, Sun Y, Abid M, Luo Y, Qiu H . Current status and evolving approaches to African swine fever vaccine development. Transbound Emerg Dis. 2019; 67(2):529-542. DOI: 10.1111/tbed.13364. View

Zhu Z, Xiao C, Fan Y, Cai Z, Lu C, Zhang G . Homologous recombination shapes the genetic diversity of African swine fever viruses. Vet Microbiol. 2019; 236:108380. PMC: 7172151. DOI: 10.1016/j.vetmic.2019.08.003. View

Petrovan V, Yuan F, Li Y, Shang P, Murgia M, Misra S . Development and characterization of monoclonal antibodies against p30 protein of African swine fever virus. Virus Res. 2019; 269:197632. DOI: 10.1016/j.virusres.2019.05.010. View

Gallardo C, Soler A, Rodze I, Nieto R, Cano-Gomez C, Fernandez-Pinero J . Attenuated and non-haemadsorbing (non-HAD) genotype II African swine fever virus (ASFV) isolated in Europe, Latvia 2017. Transbound Emerg Dis. 2019; 66(3):1399-1404. DOI: 10.1111/tbed.13132. View

Gallardo C, Fernandez-Pinero J, Arias M . African swine fever (ASF) diagnosis, an essential tool in the epidemiological investigation. Virus Res. 2019; 271:197676. DOI: 10.1016/j.virusres.2019.197676. View

Shabani Azim F, Zare Bavani M, Nikmanesh B . Luciferase Immunoprecipitation System Assay, a Rapid, Simple, Quantitative, and Highly Sensitive Antibody Detection for Parasitic Diseases. Iran J Parasitol. 2017; 11(3):426-428. PMC: 5256063. View

ODonnell V, Grau F, Mayr G, Sturgill Samayoa T, Dodd K, Barrette R . Rapid Sequence-Based Characterization of African Swine Fever Virus by Use of the Oxford Nanopore MinION Sequence Sensing Device and a Companion Analysis Software Tool. J Clin Microbiol. 2019; 58(1). PMC: 6935939. DOI: 10.1128/JCM.01104-19. View

Mirkalantari S, Amirmozafari N, Kazemi B, Irajian G . Molecular cloning of virB12 gene of Brucella melitensis 16M strain in pET28a vector. Asian Pac J Trop Med. 2012; 5(7):511-3. DOI: 10.1016/S1995-7645(12)60089-3. View

10.

Ramanathan R, Burbelo P, Groot S, Iadarola M, Neva F, Nutman T . A luciferase immunoprecipitation systems assay enhances the sensitivity and specificity of diagnosis of Strongyloides stercoralis infection. J Infect Dis. 2008; 198(3):444-51. PMC: 3379004. DOI: 10.1086/589718. View

11.

Aira C, Ruiz T, Dixon L, Blome S, Rueda P, Sastre P . Bead-Based Multiplex Assay for the Simultaneous Detection of Antibodies to African Swine Fever Virus and Classical Swine Fever Virus. Front Vet Sci. 2019; 6:306. PMC: 6753221. DOI: 10.3389/fvets.2019.00306. View

12.

Mur L, Gallardo C, Soler A, Zimmermman J, Pelayo V, Nieto R . Potential use of oral fluid samples for serological diagnosis of African swine fever. Vet Microbiol. 2013; 165(1-2):135-9. DOI: 10.1016/j.vetmic.2012.12.034. View

13.

Mur L, Igolkin A, Varentsova A, Pershin A, Remyga S, Shevchenko I . Detection of African Swine Fever Antibodies in Experimental and Field Samples from the Russian Federation: Implications for Control. Transbound Emerg Dis. 2014; 63(5):e436-40. DOI: 10.1111/tbed.12304. View

14.

Guinat C, Reis A, Netherton C, Goatley L, Pfeiffer D, Dixon L . Dynamics of African swine fever virus shedding and excretion in domestic pigs infected by intramuscular inoculation and contact transmission. Vet Res. 2014; 45:93. PMC: 4189175. DOI: 10.1186/s13567-014-0093-8. View

15.

Aye K, Nagayasu E, Baba M, Yoshida A, Takashima Y, Maruyama H . Evaluation of LIPS (luciferase immunoprecipitation system) for serodiagnosis of Toxoplasmosis. J Immunol Methods. 2018; 462:91-100. DOI: 10.1016/j.jim.2018.09.002. View

16.

Zubair A, Burbelo P, Vincent L, Iadarola M, Smith P, Morgan N . Microfluidic LIPS for serum antibody detection: demonstration of a rapid test for HSV-2 infection. Biomed Microdevices. 2011; 13(6):1053-62. PMC: 3469327. DOI: 10.1007/s10544-011-9575-x. View

17.

Wu P, Lowe A, Rodriguez Y, Murgia M, Dodd K, Rowland R . Antigenic regions of African swine fever virus phosphoprotein P30. Transbound Emerg Dis. 2020; 67(5):1942-1953. DOI: 10.1111/tbed.13533. View

18.

Ching K, Burbelo P, Gonzalez-Begne M, Roberts M, Coca A, Sanz I . Salivary anti-Ro60 and anti-Ro52 antibody profiles to diagnose Sjogren's Syndrome. J Dent Res. 2011; 90(4):445-9. PMC: 3125128. DOI: 10.1177/0022034510390811. View

19.

Xiong D, Zhang X, Xiong J, Yu J, Wei H . Rapid genome-wide sequence typing of African swine fever virus based on alleles. Virus Res. 2021; 297:198357. DOI: 10.1016/j.virusres.2021.198357. View

20.

Luong H, Lai H, Vu H . Evaluation of Antibody Response Directed against Porcine Reproductive and Respiratory Syndrome Virus Structural Proteins. Vaccines (Basel). 2020; 8(3). PMC: 7564207. DOI: 10.3390/vaccines8030533. View