Novel Picornavirus (family Picornaviridae) from Freshwater Fishes (Perca Fluviatilis, Sander Lucioperca, and Ameiurus Melas) in Hungary

Overview

Journal Arch Virol

Specialty Microbiology

Date 2021 Jul 13

PMID 34255185

Citations 8

Authors

Renata Hargitai

Peter Pankovics

Akos Boros

Robert Matics

Eda Altan

Eric Delwart

Gabor Reuter

Affiliations

Soon will be listed here.

Abstract

In this study, a novel picornavirus (perchPV/M9/2015/HUN, GenBank accession no. MW590713) was detected in eight (12.9%) out of 62 faecal samples collected from three (Perca fluviatilis, Sander lucioperca, and Ameiurus melas) out of 13 freshwater fish species tested and genetically characterized using viral metagenomics and RT-PCR methods. The complete genome of perchPV/M9/2015/HUN is 7,741 nt long, excluding the poly(A) tail, and has the genome organization 5'UTRP1(VP0-VP3-VP1)/P2(2A-2A-2B-2C)/P3(3A-3B-3C-3D)/3'UTR-poly(A). The P1, 2C, and 3CD proteins had 41.4%, 38.1%, and 47.3% amino acid sequence identity to the corresponding proteins of Wenling lepidotrigla picornavirus (MG600079), eel picornavirus (NC_022332), and Wenling pleuronectiformes picornavirus (MG600098), respectively, as the closest relatives in the genus Potamipivirus. PerchPV/M9/2015/HUN represents a potential novel fish-origin species in an unassigned genus in the family Picornaviridae.

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